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《Journal of Anhui Agricultural Sciences》 2011-25
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Effect of Transgenic Bt plus CpTI Cotton on Carboxylesterase and Acetylcholinesterase of Cotton Aphid Aphis gossypii

XUE Li et al(Department of Entomology,College of Agronomy and Biotechnology,China Agricultural University,Beijing 100193)  
[Objective] The research aimed to assess the effect of transgenic Bt plus CpTI cotton variety SGK321 on carboxylesterase and acetylcholinesterase of cotton aphid Aphis gossypii and give theoretical basis for studying the biosafety of transgenic cotton.[Method] Cotton aphids were fed with both SGK321 and Shiyuan321(normal parental varieties) for over 40 generations.Enzyme activities were compared between cotton aphids fed on SGK321 for 1,2,3,41,42 and 43 generations with those on Shiyuan321.[Result] The carboxylesterase activities of cotton aphids fed on SGK321 for 1 generation was significantly higher than those fed on Shiyuan321.Acetylcholinesterase activities of cotton aphids fed on SGK321 for 1,2 and 3 generations were significantly higher than those fed on Shiyuan321 in the same generation.But there was no significant difference of enzyme activities among cotton aphids fed on SGK321 for long run,and those on parental cotton.[Conclusion] The cotton aphid that fed on transgenic Bt plus CpTI cotton SGK321 for the long run have applicability about SGK321 through the accommodation of Detoxifying Enzyme.
【Fund】: 转基因生物新品种培育重大专项(2008ZX08012-04)
【CateGory Index】: S562
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