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《Journal of Anhui Agricultural Sciences》 2011-28
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Separation and Purification of Total Flavonoids from Passiflora edulis with Polyamide

KONG Qiu-ling et al(School of Bioscience & Bioengineering,South China University of Technology,Guangzhou,Guangdong 510006)  
[Objective] To study the separation and purification of total flavonoids from Passiflora edulis with polyamide particles.[Method] The content of total flavonoids was determined with UV spectrophotometry,and the scientific and reasonable conditions for isolating and purifying total flavonoids from Passiflora edulis with polyamide particles were obtained through observing the static,dynamic adsorption and elution conditions.[Result] Polyamide could well adsorb the flavonoids in the leaves of Passiflora edulis,the saturated static adsorption capacity was 23.14 mg/g.The dynamic adsorption and isolation conditions were:30 ml Passiflora edulis extracts were eluted with 8 BV water until without Molish reaction,then eluted with 12 BV 50% ethanol at a rate of 2 BV/h.[Conclusion] The polyamide particles had high selectivity to the adsorption of total flavonoids from Passiflora edulis,which was easy to operate,therefore it could be used to the enrichment and purification of Passiflora edulis.
【Fund】: 广东省医院药学研究基金(2011A23)
【CateGory Index】: TQ461
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