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《Journal of Anhui Agricultural Sciences》 2011-29
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The Synthesis and Identification of Copper Complete Antigen and the Preparation of Monoclonal Antibodies against Copper Ions

LIU Wen-di et al(Key Laboratory of Zoonosis Research,Ministry of Education Institute of Zoonosis,College of Animal Science and Veterinary Medicine,Jilin University,Changchun,Jilin 130062)  
[Objective] The aim was to gain the monoclonal antibody against copper ions.[Method] The carrier protein BSA,OVA were separately chelated with bifunctional chelating agent Isothiocyanobenzyl-EDTA(ITCBE) and linked with ion copper to gain the completed Immunogen(Cu-ITCBE-BSA) and the detection Immunogen(Cu-ITCBE-OVA).Non-denaturing polyacrylamide gel electrophoresis was used for the identification of the completed antigen.By using cell fusion and limiting dilution,a stable hybridoma strains secreting anti Cu2+ was obtained.[Result] A stable hybridoma strains(4A6) secreting anti Cu2+ monoclonal antibody was obtained.The sub-class of antibody was IgG1.The titer of cell culture supernatant and ascites were 1∶1024 and 1∶6.4×105 respectively.The ascites was purified by affinity chromatography,the purity was 95%.[Conclusion] A monoclonal antibody against copper ions has been obtained,which provided foundations for the detection of copper ions in environmental water samples.
【Fund】: 国家自然科学基金项目(60971011);; 吉林大学人才跟进计划项目(43050502J9)
【CateGory Index】: R392
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