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《Journal of Anhui Agricultural Sciences》 2011-30
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Study on the Enzymatic Activity of the Superoxide Dismutase of Brassica juncea Coss.var. tumida Tsen et Lee

HE Shi-min et al(Department of Life Science and Technology,Changjiang Teacher College,Fuling,Chongqing 408003)  
[Objective] The activity of SOD of Brassica juncea Cross.var.tumida Tsen et Lee and the effect of the pH value,the concentration of substrate and temperature on it were explored.[Method] The activity of SOD,the effect of the pH value,concentration of substrate and temperature on it of the tumorous stem and leaf of Brassica juncea Cross.var.tumida Tsen et Lee as experimental material were measured with the method of pyrogallicacid oxidize and the value of its developing and using was analyzed.[Result] The activity of SOD in the tumorous stem of Brassica juncea Coss.var.tumida Tsen et Lee was between 200 U/g and 300 U/g and in the leaf,between 250 U/g and 400 U/g,which indicated that the activity of SOD in the leaf was higher than that in the tumorous stem.The greatest activity of SOD in the plant was under the condition of pH between 8.3 to 8.6;under the optimum temperature of 50 ℃ and the most suitable concentration of substrate of around 7 mmol/L.[Conclusion] The activity of SOD in the leaf of tumour stem mustard was higher than that in the tumorous stem and the result could provide the reference for the development utilization of tumour stem mustard.
【CateGory Index】: S637.3
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