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《Journal of Anhui Agricultural Sciences》 2012-03
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A Preliminary Study on Callus Induction and Proliferation of Cylocarya paliurus

WANG Ji et al (College of Forest Resources and Environment,Nanjing Forestry University,Nanjing,Jiangsu 210037)  
[Objective] To carry out preliminary study on callus induction and proliferation of Cylocarya paliurus.[Method] The leaf and stem segments of Cylocarya paliurus as explants were cultured on MS,WPM and improved DKW3 mediums,added 6-BA and IBA with different concentration ratios to explore the optimum medium for callus induction and proliferation.[Result] It was found that MS+4.0 mg/L 6-BA+2.0 mg/L IBA was the optimum medium for inducing stem callus of Cylocarya paliurus,MS+2.0 mg/L 6-BA+0.5 mg/L IBA was the optimum medium for inducing leaf explants callus of Cylocarya paliurus,and MS+1.0 mg/L 6-BA+1.0 mg/L IBA was the optimum medium for callus proliferation.200 mg/L Vitamin C was best to inhibit Cylocarya paliurus callus from browning with the browning rate of only 5.71%.[Conclusion] This study provided some theoretical basis for the establishment of tissue culture and rapid propagation system and the development of molecular breeding study of Cylocarya paliurus.
【Fund】: 国家林业公益性行业科研专项(200904046);; 江苏高等学校优秀科技创新团队项目(苏教科[2009]10号);; 江苏省“六大人才高峰”资助项目
【CateGory Index】: S792.12
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