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《Journal of Anhui Agricultural Sciences》 2012-10
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Effects of Different Extraction Methods on Antioxidant Activity of Inonotus obliquus Polysaccharides

ZHANG Li-xia et al(School of Life Sciences,Daqing Normal College,Daqing,Heilongjiang 163712)  
[Objective] This study aimed to explore the effects of different extraction methods on antioxidant activities of I.obliquus polysaccharides.[Method] Crude polysaccharides were extracted by using boiling ethanol precipitation method and ethanol-alkali extraction method;water-soluble and alkali-soluble polysaccharides were purified by using trichloroacetic acid method;antioxidant activities of I.obliquus polysaccharides were compared by hydroxyl radical scavenging experiment and superoxide anion radical scavenging experiment.[Result] Yield of crude water-soluble polysaccharides,crude alkali-soluble polysaccharides,pure water-soluble polysaccharides and pure alkali-soluble polysaccharides were 14.0%,27.7%,7.0% and 6.2%,respectively.Hydroxyl radical scavenging effect of crude water-soluble polysaccharides and pure alkali-soluble polysaccharides had achieved the maximum with polysaccharide concentration of 0.9 mg/ml and 0.06 mg/ml,and the scavenging rates were 54.3% and 10.9%,respectively;superoxide anion radical scavenging effect of crude water-soluble polysaccharides and pure water-soluble polysaccharides had achieved the maximum with polysaccharide concentration of 0.1 mg/ml and 0.07 mg/ml,and the scavenging rates were 24.2% and 27.2%,respectively.[Conclusion] Antioxidant activity of water-soluble polysaccharides was higher than that of alkali-soluble polysaccharides.I.obliquus could be used as a species of new efficient natural antioxidant medicinal fungi in application.
【Fund】: 黑龙江省教育厅科学技术研究指导项目(11533005)
【CateGory Index】: S646
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