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《Journal of Anhui Agricultural Sciences》 2012-10
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Effects of Different Extraction Methods on Antioxidant Activity of Inonotus obliquus Polysaccharides

ZHANG Li-xia et al(School of Life Sciences,Daqing Normal College,Daqing,Heilongjiang 163712)  
[Objective] This study aimed to explore the effects of different extraction methods on antioxidant activities of I.obliquus polysaccharides.[Method] Crude polysaccharides were extracted by using boiling ethanol precipitation method and ethanol-alkali extraction method;water-soluble and alkali-soluble polysaccharides were purified by using trichloroacetic acid method;antioxidant activities of I.obliquus polysaccharides were compared by hydroxyl radical scavenging experiment and superoxide anion radical scavenging experiment.[Result] Yield of crude water-soluble polysaccharides,crude alkali-soluble polysaccharides,pure water-soluble polysaccharides and pure alkali-soluble polysaccharides were 14.0%,27.7%,7.0% and 6.2%,respectively.Hydroxyl radical scavenging effect of crude water-soluble polysaccharides and pure alkali-soluble polysaccharides had achieved the maximum with polysaccharide concentration of 0.9 mg/ml and 0.06 mg/ml,and the scavenging rates were 54.3% and 10.9%,respectively;superoxide anion radical scavenging effect of crude water-soluble polysaccharides and pure water-soluble polysaccharides had achieved the maximum with polysaccharide concentration of 0.1 mg/ml and 0.07 mg/ml,and the scavenging rates were 24.2% and 27.2%,respectively.[Conclusion] Antioxidant activity of water-soluble polysaccharides was higher than that of alkali-soluble polysaccharides.I.obliquus could be used as a species of new efficient natural antioxidant medicinal fungi in application.
【Fund】: 黑龙江省教育厅科学技术研究指导项目(11533005)
【CateGory Index】: S646
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【References】
Chinese Journal Full-text Database 2 Hits
1 ZHAO Chen-hao,LIU Jun-fa,FENG Meng-ying,WEN Ling-rong,YOU Li-jun,ZHAO Mou-ming(College of Light Industry and Food Sciences,South China University of Technology,Guangzhou 510640,China);Effect of Different Extraction Methods on the Properties of Longan Polysaccharides[J];Modern Food Science and Technology;2012-10
2 JIN Li-na1,2,DONG Fang1,2,LIU Zhen-feng1,2,HAO Ying1,2,JI Yu-bin1,2,3(1.Center of Life Science and Environmental Science,Harbin University of Commerce; 2.Engineering Research Center of Natural Anti-tumor Drugs,Ministry of Education; 3.Post-doctoral Research Center of Drugs Research Institute:Harbin 150076,China);Study on the Extraction Methods of Bioactive Polysaccharide[J];Journal of Binzhou University;2012-06
【Citations】
Chinese Journal Full-text Database 4 Hits
1 YAN Jun,GUO Xiaoqiang,Wu Xiaoyong,XU Guangyu,GOU Xiaojun(The Laboratory of Chinese Traditional Medicine,Chengdu University,Chengdu 610106,China);Research on the Ability to Scavenge Free Radical of Tremella Polysaccharide[J];Journal of Chengdu University(Natural Science Edition);2006-01
2 LIN Li-qing,HUANG Li-ying,ZHENG Yan-jie,et al(School of Pharmacy,Fujian Medical University,Fuzhou,Fujian 350004,China);Study on Extraction of Polysaccharide from Anoectochilus formosanus and Its Effect of Removal of Oxygen Free Radicals[J];Journal of Fujian College of Traditional Chinese Medicine;2006-05
3 JIN Guang1, YANG En-yue1, JIN Qing-hao2, HONG Chun-zan3(1.Department of Pharmacology, Yanbian University College of Medicine , Yanji 133000, Jilin, China;2.Department of Pharmacy, Yanbian Universi ty College of Pharmacy, Yanji 133000, Jilin, China;3.Department of Pharmacy, Aff iliated Hospital of Yanbian University College of Medicine, Yanji 133000, Ji lin, China);Studies on antitumor activities of Fuscoporia obique polysac charide[J];Journal of Medical Science Yanbian University;2004-04
4 YOU Yu hong, LIN Zhi bin (Department of Pharmacology, School of Basic Medical Sciences, Health Science Center, Peking University, Beijing 100083, China);Antioxidant effect of Ganoderma polysaccharide peptide[J];Acta Pharmaceutica Sinica;2003-02
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 LI Jian-guang et al (College of Food and Biology Engineering, Zhengzhou University of Light Industry, Zhengzhou, Henan 450002);Research Review on Inonotus obliquus[J];Journal of Anhui Agricultural Sciences;2008-02
2 CHEN Xue-feng et al (College of Life Science and Engineering, Shaanxi University of Science and Technology, Xi'an, Shaanxi 710021);Study on the Activity of Scavenging Free Radicals of Nostoc flagelliforme Polysaccharides[J];Journal of Anhui Agricultural Sciences;2008-08
3 BI Yong-guang et al(College of Pharmacy,Guangdong Pharmaceutical University,Guangzhou,Guangdong 510006);Study on the Scavenging Activities of Extracts from Lygodium japonicum(Thunb)Sw.with Different Solvents to Free Radicals[J];Journal of Anhui Agricultural Sciences;2009-19
4 CHEN Yi-yong et al(State Key Laboratory of Food Science and Technology,Jiangnan University,Wuxi,Jiangsu 214036);Study on the Ultrasonic Microwave Assisted Extraction Technology of Polysaccharides from Inonotus obliquus[J];Journal of Anhui Agricultural Sciences;2009-31
5 ZHANG Li-xia et al(School of Life Science,Daqing Normal University,Daqing,Heilongjiang 163712);Study on the Microwave-assisted Extraction and Antioxidation of Polysaccharides from Beer Yeast[J];Journal of Anhui Agricultural Sciences;2011-11
6 HU Tao et al(College of Food science & Nutritional Engineering,China Agricultural University,Beijing 100083);Study on the Ultrasonic Assisted Extraction Technology of Triterpenoid from Inonotus obliquus[J];Journal of Anhui Agricultural Sciences;2011-35
7 JI Hong1,2,3,CHEN Wen-jie2,MA Yun-li3,HAN Tao2,HUO Hong2(1.Changshu Institute of Technology,Changshu,Jiangsu 215500,China;2.Hebei Institute of Microbiology,Baoding,Hebei 071051,China;3.TianJin University,Tianjin 300072,China;4.Hebei Agriculture University,Baoding,Hebei 071051,China);Study on Domestication and Cultivation of Wild Inonotus obliquus Strain JS-1[J];Northern Horticulture;2009-02
8 LI Yu-wei,LIAN Rui-li,BAN Qiang,CHEN Zhong-jie,CHENG Mao-gao,LI Xian-fang(Department of Medicinal Engineering,Zhengzhou College of Animal Husbandry and Engineering,Zhengzhou,Henan 450011);Optimization of Liquid Fermentation Conditions for Ganoderma Lucidum Using Response Surface Methodology[J];Northern Horticulture;2012-13
9 Han Jiangyu,Zhang Guangji,Shi Senglin Zhejiang University of Traditional Chinese Medicine,Hangzhou (310053);Evaluation on Recent Situation of Plant-polysaccharide Anti-tumor Research[J];Journal of Zhejiang University of Traditional Chinese Medicine;2008-02
10 ZHAO Jun1,2 JIN RiGuang1 LUO Lu1 (1.College of Materials Science and Engineering,Beijing University of Chemical Technology,Beijing 100029;2.National Institute of Pharmaceutical Research & Development Co Ltd,Beijing 102206,China);A correlation between the anti-HIV properties of Chinese medicinal herbs and their trace element content[J];Journal of Beijing University of Chemical Technology(Natural Science Edition);2007-05
China Proceedings of conference Full-text Database 1 Hits
1 ZHANG Zhen-Yu TIAN Xue-Mei BU Xiang-Hui SONG Ai-Rong~* (Shandong Provincial Key Laboratory of Applied Mycology Qingdao Agricultural University,Qingdao 266109, China);Reseach on anti-tumor activity in vitro of extracts from Inonotus obliquus[A];[C];2011
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 MA Hong-bo et al(Department of Nutrition,Jilin Medical College,Jilin,Jilin 132013);Extraction of Polysaccharide form Mulberry Leaf by Sodium Hydroxide and Content Determination[J];Journal of Anhui Agricultural Sciences;2011-03
2 CHEN Ming et al(College of Resource and Environmental Engineering,Jiangxi Polytechnic University,Ganjiang,Jiangxi 341000);Progress of Extraction Technoloqy of Tea Polysaccharide and Study on Supercritical Fluid Extraction[J];Journal of Anhui Agricultural Sciences;2011-08
3 XIE Ai-ze et al(National Medical Research Institute of Guangxi,Nanning,Guangxi 530001);Study on the Extraction of Polysaccharides from Camellia chrysantha(Hu) Tuyama by Acetone Precipitation[J];Journal of Anhui Agricultural Sciences;2011-25
4 ZENG Hong-liang,LU Xu,BIAN Zhen-yu,LIN Yu-fei,ZHANG Yi(College of Food Science,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China);Optimization of the extraction technique of Fortunella margarita polysaccharides via response surface analysis[J];Journal of Fujian Agriculture and Forestry University(Natural Science Edition);2012-03
5 PENG Chuan-Cong1,KONG Jing2,YOU Li-Jun2,MA Fang-Li1(1.Infinitus(China) Company Ltd,Guangzhou 510665,China)(2.College of Light Industry and Food Sciences,South China University of Technology,Guangzhou 510640,China);Optimization of Ultrasonic-assisted Extraction Technology of Lentinan Polysaccharides by Response Surface Methodology and its Antioxidant Activity[J];Modern Food Science and Technology;2011-04
6 CHEN Yao-hua,CHEN Jian(College of Light Industry and Food Sciences,South China University of Technology,Guangzhou 510640,China);Optimization of Extraction Conditions for Polysaccharides from Collybia albuminosa via Response Surface Methodology[J];Modern Food Science and Technology;2012-05
7 JIAO Guang-lian,YANG Yan,HE Bao-hua(Research Center of Membrane Separation Engineering Technology of Gansu Province,R&D Center,Gansu Academy of Membrane Science & Technology,Lanzhou 730000,China);Research on the Extraction of Astragalus Polysaccharides by Ultrafiltration[J];Chemistry & Bioengineering;2010-08
8 LIANG Mina,ZOU Dong-huib,GUO Hong-wenb,LI Angb(a.College of Chemistry and Chemical Engineering;b.School of Food and Bio-Engineering,Qiqihar University,Qiqihar 161006,Heilongjiang,China);Combined-Enzyme Extraction and Spectral Analysis of Flammulina velutipes Polysaccharides[J];Hubei Agricultural Sciences;2012-06
9 DING Chang-ling SUN Xiu-tao WANG Fei-jiu LIANG Zhou-rui WANG Wen-jun LI Tao (Key Laboratory for Sustainable Utilization of Marine Fisheries Resources,Ministry of Agriculture, Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Qingdao 266071);Extraction of polysaccharides from Sargassum thunbergii by cellulase hydrolysis and ultrasound[J];Progress in Fishery Sciences;2011-06
10 TIAN Luo~1,XUAN Yi-fang~1,FAN Rong-jun~1,JIANG Yun-lei~2,XING Ming~1,LIU Li-dong~1,CHEN Xia~1(1.College of Life Science,Jilin University,Changchun 130021,China;2.College of Animal Technology,Jilin Agricultural University,Changchun 130018,China);Ethanol-alkali Extraction Method of Astragalus Polysaccharides[J];Journal of Jilin University(Science Edition);2006-04
【Secondary References】
Chinese Journal Full-text Database 1 Hits
1 WANG Yurong;JIN Yulan;PIAO Meizi;REN Hang;College of Chemistry and Pharmaceutical Sciences, Qingdao Agricultural University;College of Food Science and Engineering, Qingdao Agricultural University;Orange Resources R&D (Qingdao) Co.,Ltd.;;Isolation and chemical characterization of polysaccharide from hawthorn[J];China Brewing;2013-09
【Secondary Citations】
Chinese Journal Full-text Database 8 Hits
1 WU Wu-tong CHEN Qiong-hua (Nanjing College of Pharmacy) XU Bi-ru WANG Sai-zhen HHEN Jin-tu (Sanming Fungi Research Institute in Fujian, Sanming);ISOLATION AND ANALYSIS OF POLYSACCHARIDE FROM THE SPORES OF TREMELLA FUCIFORMIS[J];Mycosystema;1982-02
2 XIAO Hua Shan, HE Wen Jin, FU Wen Qing, CAO Hong Yun, FAN Zi Nan( Biotechnology College of Fujian Normal University ,Fuzhou 350007,China).;A Spectrophotometer Method Testing Oxygen Radicals .[J];PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS;1999-02
3 PANG Zhan Jun * , CHEN Yuan and ZHOU Mei ( Research Laboratory of Free Radical Medicine, The First Military Medical University, Guangzhou 510515, China );The Effect of Polysaccharide Krestin on GPx Gene Expression in Macrophages[J];ACTA BIOCHIMICA ET BIOPHYSICA SINICA;1999-03
4 FANG Yun-Zhong 1, YANG Sheng 2, WU Guoyao 3 ( 1Department of Biochemistry and Molecular Biology,Beijing Institute of Radiation Medicine, Beijing 100850; 2Division of Animal Nutrition, Department of Animal Sciences, China Agricultural University, Beijing 100094; 3Department of Animal Science and Faculty of Nutrition, Texas A & M University, College Station, Texas, USA 77843);Free Radical Homeostasis[J];Progress In Physiological Sciences;2004-03
5 YAN Jun,XU Guang-yu,GUO Xiao-qiang,LIU Wei,LI Xiao-guang,GOU Xiao-jun* (The Laboratory of Traditional Chinese Medicine of Chengdu university, Chengdu 610106, China);Research on the Purification and Anti-Oxidize Activity of Tremella Polysaccharide[J];Food Science;2005-09
6 Wang Tao;Liu Chunjie;Shi Jun et al(Department of Oncology, First Affiliated Hospital);SERUM LEVELS OF LIPID-BOUND SIALIC ACID AND TUMOUR NECROSIS FACTOR IN PATIENTS WITH LUNG CANCER BEFORE AND AFTER RADIOTHERAPY[J];JOURNAL OF XI'AN MEDICAL UNIVERSITY(CHINESE);1998-02
7 CHEN Jiong-ran~1, WANG Qin~2 (1. Lanzhou Institute of Animal Science and Veterinary Pharmaceutics, Chinese Academy of Agricultural Sciences, Lanzhou 730050, China; 2. College of Life Science, Lanzhou University, Lanzhou 730000,China);Extraction of Potentilla anserine polysaccharide and its effects of scavenging oxygen free radicals[J];Chinese Journal of Veterinary Science and Technology;2004-04
8 Jin Juqing,Bian Xiaoli,Ge Ping, Jing Hui,Ding Ling, Ding Dongling (Xi'an Jiao Tong University, Xi'an 710061);The Effects of the Polysaccharides from Dermatocarpon miniatum on Oxygen Radicals and Lipid Peroxidation[J];Journal of Chinese Medicinal Materials;2001-09
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