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《Journal of Anhui Agricultural Sciences》 2012-13
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Extraction and Content Determination of Polysaccharides from Neverita didyma

YANG Hong-li et al(College of Food and Pharmaceutical Science,Zhejiang Ocean University,Zhoushan,Zhejiang 316000)  
[Objective] To optimize the extraction conditions and determine the content of polysaccharide from Neverita didyma.[Method] The polysaccharide content was determined by phenol-sulfuric acid method,with the yield of polysaccharide as index,an orthogonal analysis was carried out based on single factor analysis to optimize the extraction conditions of polysaccharide from Neverita didyma.[Result] The optimum extraction parameters were:extraction temperature of 40 ℃,extraction duration of 60 min,solid-liquid ratio of 1:100.Under the best conditionsy the extraction rate of Neverita didyma polysaccharide was 7.17% with RSD of 1.05%.[Conclusion] The method is simple and reliable,thus providing basis for the further development and utilization of Neverita didyma polysaccharide.
【Fund】: 浙江省科技厅(2011C31016)
【CateGory Index】: S917.4
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