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《Journal of Anhui Agricultural Sciences》 2012-16
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Study on SSR Markers Linked to Flesh Color around the Stone of Prunus persica(L.) Batsch

HAN Ji-cheng et al(College of Horticulture,Northeast Agriculture University,Harbin,Heilongjiang 150051)  
[Objective] This study aimed to select SSR molecular markers linked to flesh color around the stone of Prunus persica(L.) Batsch.[Method] P.persica(L.) Batsch varieties Chongyanghong and Jinbao were used as parents to construct F1 orthogonal group.138 F1 individuals were selected as experimental materials for construction of color around the stone gene pool(B1) and non-color around the stone gene pool(B2) by using bulked segregant analysis(BSA) method,molecular markers linked to the flesh color around the stone of P.persica(L.) Batsch were selected with SSR molecular marker technology.[Result] After selection with 256 pairs of SSR primers,three pairs of molecular markers linked to the gene controlling flesh color around the stone of P.persica(L.) Batsch were selected(UDP96-003,ch04g09 and UDP97-402).In addition,genetic distances of the three molecular markers with the gene controlling flesh color around the stone of P.persica(L.) Batsch were calculated,which were 16.7,10.1 and 17.0 cM,respectively.[Conclusion] This study laid the foundation for further selection of co-dominant molecular markers with closer genetic distance.
【Fund】: 河北省农林科学院资助项目(A06120203)
【CateGory Index】: S662.1
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