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《Journal of Anhui Agricultural Sciences》 2012-17
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Isolation and Purification of Gardenia Yellow Pigment by Macroporous Resin HPD-600

GAO Li et al(College of Life Science and Technology,Xiaogan University,Xiaogan,Hubei 432000)  
[Objective] To study the absorption and purification conditions of Gardenia yellow pigment by macroporous resin.[Method] The best macroporous resin was screened out from nine resins according to their adsorption and elution performance of Gardenia yellow pigment.The influence of temperature,acidity,etc.on the absorption and desorption of HPD-400 was investigated to explore its best isolation and purification conditions.[Result] HPD-400 showed the largest adsorption capacity,its best adsorption conditions were 25 ℃ temperature,pH 3.0 and the concentration of crude extracts A440nm=0.590;the best elution conditions were 70% ethanol concentration and 25 ℃ temperature.[Conclusion] HPD-400 shows the best isolation and purification effects of Gardenia yellow pigment,thus can be used in industrialized production.
【Fund】: 湖北省自然科学基金重大项目 鄂财企发[2009]91号第931号
【CateGory Index】: TS264.4
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