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《Journal of Anhui Agricultural Sciences》 2013-06
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Study on the Hemolysin Activity in Serum of Macrobrnahium nipponese

CHU Yan-liang et al(College of Biological and Chemical Engineering,Jiangsu University of Science and Technology,Zhenjiang,Jiangsu 212018)  
[Objective] The research aimed to provide scientific basis and basic materials for further understanding the immunologic mechanism of shrimp.[Method]Using Macrobrnahium nipponese as test materials,the hemolytic activity and glycoinhabiting action in the serum were determined by hemolysis test and glycoinhabiting test.And the effects of external factors on the hemolytic activity were analyzed.[Result] The hemolysin in the serum of M.nipponese showed higher sensitivity to erythrocytes of fish and chicken.D-glucose had promotion on hemolysin in the serum of M.nipponese,but D-mannose,D-sorbose,α-lactose,L-rhamnose and D-arabinose had inhibitory effects.The hemolysis of hemolysin at 50-60 ℃ was stronger,but it denatured and lost its hemolytic activity above 70 ℃.Hemolysin had wide pH range,and it had hemolytic effects on chicken erythrocytes in the range of pH 2-12.And the hemolytic effects was better in pH 6-9.The optimum salinity range was 3-5.Hemolysin was sensitive to Cu 2 + and its activity was completely inhibited.Methamidophos and dipterex could affect hemolysin activity.The flavonoids from Humulus scandens,Zingiber officinale and polysaccharides from H.scandens and Morus alba L leaves could enhance the hemolytic activity in the serum of M.nipponese.[Conclusion] The hemolytic activity in the serum of M.nipponese could be influenced by environmental factors and had certain stability.And hemolysin was an important component of shrimp’s immune system.
【CateGory Index】: S917.4
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