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《Journal of Anhui Agricultural Sciences》 2013-14
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Study on the Anticancer Activity of the Ruditapes philippinarum Hydrolysate on the Lung Cancer H1299 cells

LI Li et al ( Zhejiang Provincial Key Engineering Technology Research Center of Marine Biomedical Products,School of Food Science and Pharmacy,Zhejiang Ocean University,Zhoushan,Zhejiang 316004)  
[Objective]To discuss the preparation of peptide from Ruditapes philippinarum and anti-lung cancer H1299 cells activity in vitro.[Method]Trypsin was used to enzymolysis,3 kD molecular weight cut-off membranes were used to fractionate the Ruditapes philippinarum hydrolysate,and 3 kD weight fractions were obtained.The anti-proliferation activity and the early apoptosis of Ruditapes philippinarum peptides were detected by MTT,HE staining and AO / EB double staining,respectively.[Result]MTT assay showed that the peptide inhibited proliferation of H1299 cells in both concentration-dependent and time-dependent manners;Cell apoptosis morphological feature was observed under HE staining and AO / EB double staining,and staining showed that the peptide induced cellular morphological change.[Conclusion]The Ruditapes philippinarum hydrolysate inhibited the lung cancer H1299 obviously and induction of apoptosis.
【Fund】: 浙江省自然科学基金(LY12C20008 LY12C2005)
【CateGory Index】: S917.4
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