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《Journal of Anhui Agricultural Sciences》 2014-01
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Study on the Influence of Simulated Acid Rain on Clover

HU yan-chun;Neijiang Vocational & Technical College;  
[Objective]To provide sicentific basis management for the clover as ground cover plants in the acid rain areas though this experiment.[Method] In the experiment,we treated the clover with simulated acid rain at pH 2.5,3.0,3.5,4.0,4.5,6.5,and studied the germination rate,germination energy of the seed and the protein content,the malondialdehyde(MDA) content,proline acid content,and peroxidase(POD) activity changes of the seeding.we used Coomassie brilliant blue G-250 staining test the protein content,thiobarbituric acid(TBA) test the malondialdehyde(MDA) content,Acidic ninhydrin colorimetric method test the proline acid content,Colorimetry test the POD activity.[Result]At different pH values under the influence of acid rain,clover seed germination,germination rate,germination index and seedling protein content decreased as the pH decreased;malondialdehyde(MDA),proline content and over peroxidase(POD) activity decreased as the pH of the upward trend.[Conclusion]Acid Rain Stress under pH 3.5 have obvious impact on the germination and seedling physiological indices of the clover.
【CateGory Index】: S541.2
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