WuCai (B. Campestris L. ) Tissue Culture and Heat Resistant Mutant Selection
Chen Jingxian;Nie fan;Pan Miafang (Horticulture Institute,Anhui Academy of Agricultural Sciences. Hefei 230031)
Cultured on MS medium with hormones for 25 days,hypocotyl and cotyledon of Wucai formed callus,but frequency of callus was 75. 5%,and stem tip straightly formed bud cluster. Callus cultured on MS medium cantaining singBAL-1 could differentiate into plantlets and differentiation frequency was only 0. 5%. 10. 6 per cent of planls could survivethrugh high temperature for the plantiets and bud cluster. These heat-resistant plants could mormally grow at 35 ℃,buttheir fruiting rates were lower. Only 30% of plants were fertile. Heat-resistant' stability and quality of generation plantprogeny are being studied.
【CateGory Index】： S634.903.5