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《Journal of Anhui Agricultural Sciences》 1997-04
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Studies on the Detection for Duck Hepatitis Virus by Dot Immuno-gold Filtration Assay

Zhang Xiaofei et al(Animal Husbandry and Veterinary Research Institute. Anhui Academy of Agricultural Sciences,Hefei 230031)  
The purified rabbit anti-DHV IgG was labelled with the prepared colloidal gold. DIGFA with a microfilter paper as carrier and the red colloidal gold as label was developed for detection of duck hepatitis virus(DHV).The optimum working concentration of DHV antigen and rabbit anti-DHV IgG labelled with colloidal gold were determined to be 1∶320 and 1 ∶4.The detection of DIGFA had the advantages of qualitaive and quantitative analysis.The minimum amount of purified DHV antigen by the DIGFA was 4.12ng per dot,with the sensitivity of 2-fold more than the antigen spot test(AST).The high specificity of the DIGFA was shown by the specific blocking test of anti-DHV serum and the cross-rection test with DPV,GPV,NDV,IBDV,MDV,EDS76V and IBV.The positivc rate for allantoic fluid of experimentally inoculatcd embryonated eggs was 100%,The positive rate of 36 clinical duckliver samples measured by the DIGFA and AST were 83.3%and 75%,respectively. The agreement rate of DHV detected by the DIGFA and AST was 86.7%.Many virus particles were observed about 30 ̄40nm diameter by electromicroscope from specimens which were obtained by experimental infection with 6 random DIGFA-positive duck liver asmples. This study indicated that the DIGFA was a simple,trace,rapid,sensitive and specific method for the detection of DHV antigen.
【Fund】: 农业部“九五”畜牧重点项目
【CateGory Index】: S854.4
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