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《Journal of Anhui Normal University(Natural Science)》 2010-03
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In Vitro Embryo Culture of Taxus chinensis var. mairei

YANG Fei1,ZHANG Xiao-ping1,SUN Qi-wu2(1.College of Life Sciences,Anhui Normal University,Wuhu 241000,China;2.Research Institute of Forestry,Beijing 100091,China)  
Four basal media were used to investigate the effects of media on the germination and growth of isolated mature zygotic embryos of Taxus chinensis var.mairei(Lemée Lévl.) S.Y.Hu ex Liu cultured in continuous darkness and under 10-photoperiod.The results showed that germination percentage of isolated embryos was significantly affected by the culture medium with B5 medium giving the highest of 96%.The time required for isolated embryos to germinate varied with different media.Light resulted in higher germination frequency in comparison with dark.The formulation of the basal medium had an apparent effect on the growth of germinated embryos.B5 medium was approved to be optimal for early seedling growth with the highest survival rate(70%) and best rooting result.In this study we provided an efficient method for overcoming seed dormancy and also for rapid propagation and conservation and taxol production of T.mairei.
【Fund】: Supported by Protection Reproduction and Sustainable Utilization of Taxus Plant Project of China(2130211)
【CateGory Index】: S791.49
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