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《Journal of Anhui Normal University(Natural Science)》 2011-03
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Study on Extraction of Genomic DNA from Pteroceltis tatarinowii Maxim

ZHANG Hui,ZHANG Xiao-ping,LI Xiao-hong,CHEN Wen-long(Key Laboratory for the Conservation and Utilization of Important Biological Resources in Anhui Province,Anhui Normal University,Wuhu 241000,China)  
The genomic DNA was isolated from the leaf of Pteroceltis tatarinowii Maxim.by using the improved high salt and low pH method,improved SDS method,improved CTAB method and genomic DNA kit.The quality of the DNA was examined by agarose gel electrophoresis,spectrophotometry and PCR amplification of ISSR.The result showed that the method of improved CTAB was obviously better than the others.The DNA sample obtained by the improved CTAB method was pure and had good quality which could satisfy the need for further analysis.
【Fund】: 国家自然科学基金(30840020;30970292)
【CateGory Index】: Q943.2
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