Initial Observation on DNA Trauma of Fish Red Blood Cell Caused by organic distill from Chaohu Lake
Zhao Ying, Wang Zhi-qiang, Yang Zhi-ping, Wang Wei, Huang Xiao-mu (Anhui Center for Disease Control and Prevention ,Hefei,Anhui,230061,China)
Objective To study mutagenicity of organic distill from Chaohu lake. Methods Single cell gelatin electrophoresis test(SCGE Test) was used to measure DNA trauma of fish red blood cell. Results the percent of comet cell caused by source water of Chaohu lake was the highest(57.25%),and the percent before the water filtered was the lowest(27.63%),the percent of comet cell in leaving water went up to 44.00% by chlorinating two times. Conclusions Source water of Chaohu lake had latent mutagenicity, the DNA trauma of fish red blood cell could be decreased by coagulative precipitation, absorption of active carbon and precipitation, but the action might be amplified by adding chlorine.