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《Anhui Medical Journal》 2007-01
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Analysis of the influence of direct bilirubin and indirect bilirubin on enzymatic measurement of triglyceride

GAO Yong Department of Clinical Laboratory,The Second Hospital of FuYang,AnHui,FuYang 236015  
Objective To examine the effect of different kinds of bilirubin that include direct bilirubin and indirect bilirubin on the direct enzymatic measurement of triglyceride(TG)by using the TG and peroxidase couple reaction.Methods we prepared much mixed sera of the different level of direct bilirubin(DBIL)and indirect bilirubin(IBIL),and they were divided three groups of high、middle and low according to the level of TG.The level of DBIL and IBIL were measured by HITACHl 7170A automatic analyzer,the level of TG of each serum sample was measured by using both the direct enzymatic measurement and acetic acetone measurement.Results DBIL produced greatest negative interference,when concentration of DBIL was 280 μmol/L,recovery rate of TG was only 62.8%、60.3%、58.8%,but the effect of IBIL was slight,when concentration was 240 μmol/L,recovery rate of TG was over 90%.Conclusion The DBIL and IBIL produced different interference in the enzymatic measurement of TG,They should be distinguished in our every day work.Especially,the interference produced by the higher lever of DBILwas greater,was adjusted in the result of checking.
【CateGory Index】: R446.11
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