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Content determination of syringin in Mie'aoling tablet by HPLC

ZHAO Cheng(Luan Institute for Drug Control,Anhui Province 237000,China)  
Aim To establish a method for the determination of syringin in Mie'aoling tablet.Methods HPLC method was used.The samples were separated by VP-ODS column(150 mm×4.6 mm,5 μm);The mobile phase is methanol-water(20∶80).The detection wavelength was 265 nm,with flow rate at 1.0 ml·min~(-1).The column temperature was indoor temperature.Results The linear range of syringin was 0.106~1.06 μg(r=0.9996,n=6).The average recovery was 99.43%,and RSD was 1.61%.Conclusion This method is simple,sensitive,accurate and can be used to determine the contents of syringin in Mie'aoling tablet.
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