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《Anhui Medical and Pharmaceutical Journal》 2007-05
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Optimization of alcohol precipitation techniques for polysaccharides from Armillariella tabescens by orthogonal test

ZHANG Ping1,2,SONG Qun-liang2,HUANG Zhao-gang1,LIU Li-Ping1,SUN Xu-Qun1,CHENG Gang1(1.Department of Pharmacy,The First Affiliated Hospital of Anhui Medical University,Hefei 230022;2.Department of Pharmacy,Anhui Medical University,Hefei 230032)  
Aim To determine the alcohol precipitation techniques for polysaccharides from Armillariella tabescens.Method The alcohol precipitation process was studied with yield and content of poysaccharide by orthogonal test.Result The optimum process was determined as following:the solution was concentrated to 1/6 g Armillariella tabescens per milliliter and precipitated with the alcohol concentration of 80% and the time of 6 hours by driping.Conclusion The optimum alcohol precipitation process was simple,resonable and reliable.
【Fund】: 安徽省教育厅自然科学基金项目(No2002kj138)
【CateGory Index】: TQ461
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