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《Anhui Medical and Pharmaceutical Journal》 2007-06
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Determination of polysaccharide in Armillariella tabescens by phenol-sulfuric acid colorimetry

SONG Qun-liang1,ZHANG Ping1,2,SUN Xu-qun2,LI Shao-ping2,HUANG Zhao-gaog2,XIA Quan2(1.Department of Pharmacy,Anhui Medical University,2.The First Affiliated Hospital of Anhui Medical University,Hefei 230032)  
Aim To establish a simple and accurate method for the determination of polysaccharide in Armillariella tabescens.Method Phenol-sulfuric acid colorimetric analysis is employed.Result The detected wavelength was 490 nm.Linear range was 3.1~15.6 mg·L-1.The average recovery rate was 99.78% and RSD was 0.99%.The polysaccharide content of Armillariella tabescens in total polysaccharide was 68.72% and RSD was 1.28%.Conclusion The method is convenient,rapid,accurate and reproducible.It can be used for quality control of polysaccharide of Armillariella tabescens.
【Fund】: 安徽省教育厅自然科学基金项目(No2002kj138)
【CateGory Index】: R284
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