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《Anhui Medical and Pharmaceutical Journal》 2007-08
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Determination of betaine in lycium Chinese by HPLC

ZHEN Lu-xu,WU Hai-lin,FANG Zong-hua,XU Le,JIANG Zhong-zan(East China Traditional Chinese Medicine Engineering Group Co.,LTD,Hefei 230088)  
Aim To establish a method for determination of betaine in lycium Chinese by HPLC.Methods The chromatographic column was shim-park Hypersil SCX(250 mm×4.6 mm,5.0 μm).The mobile phase was acetonitrile-0.05 mol·L-1potassium dihydrogen phosphate-phosphoric acid(15∶85∶0.18),and UV detection was performed at 192 nm.Results The linear range was 0.40~2.40 μg,the average recover was 98.99%,and RSD was 0.86%(n=6).Conclusion This method is accurate,reliable as well as specific and can be used to determine the content of betaine in lycium Chinese.
【CateGory Index】: R284
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