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《Anhui Medical and Pharmaceutical Journal》 2007-12
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Removal of proteins from crude astragalus polysaccharides by sevag method

XIA Quan1,LIU Gang2,GE Chao-liang1,XU Du-juan1(1.Department of Pharmacy,The First Affiliated Hospital of Anhui Medical University,Hefei 230022;2.School of Pharmacy,Anhui Medical University,Hefei 230032)  
Aim To study the effect of removing proteins from crude astragalus polysaccharides by sevag method.Methods Sevag method was adopted to remove proteins.Coomassie brilliant blue method and phonel-sulfuric acid method were used to determine the content variation of proteins and polysaccharides before and after Sevag method.Results 70.8% of proteins were removed by sevag method after treatment for 6 times while little change of the content of polysaccharide(only reduced by 4.44%) was found.Conclusion Sevag method can be used effectively to remove proteins from crude astragalus polysaccharide as well as an effective purification method.
【Fund】: 安徽省自然科学基金项目(070413067);; 安徽省教育厅自然科学研究项目(KJ2007B149)
【CateGory Index】: R284
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