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《Anhui Medical and Pharmaceutical Journal》 2007-12
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Removal of proteins from crude astragalus polysaccharides by sevag method

XIA Quan1,LIU Gang2,GE Chao-liang1,XU Du-juan1(1.Department of Pharmacy,The First Affiliated Hospital of Anhui Medical University,Hefei 230022;2.School of Pharmacy,Anhui Medical University,Hefei 230032)  
Aim To study the effect of removing proteins from crude astragalus polysaccharides by sevag method.Methods Sevag method was adopted to remove proteins.Coomassie brilliant blue method and phonel-sulfuric acid method were used to determine the content variation of proteins and polysaccharides before and after Sevag method.Results 70.8% of proteins were removed by sevag method after treatment for 6 times while little change of the content of polysaccharide(only reduced by 4.44%) was found.Conclusion Sevag method can be used effectively to remove proteins from crude astragalus polysaccharide as well as an effective purification method.
【Fund】: 安徽省自然科学基金项目(070413067);; 安徽省教育厅自然科学研究项目(KJ2007B149)
【CateGory Index】: R284
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2 QU Hong-wei et al(College of Life Sciences,Yantai University,Yantai,Shandong 264005);Study on Bacteriostasis and Anti-oxidation of Polysaccharides from Ophiopholis mirabilis[J];Journal of Anhui Agricultural Sciences;2013-15
3 ZHENG Li1,HAN Ping1,LIU Tao2,CUI Zhi-song1,GUO Xiu-chun1,LUO Dan2,GAO wei1(1.The First Institute of Oceanography,State Oceanic Administration,Qingdao 266061,China;2.Ocean University of China,Qingdao 266003,China);Fluorescence quantitative detection and extraction process optimization of laminarin from kelp[J];Marine Sciences;2012-05
4 Han Wei Huang Xi Zhang Lingling Lü Jiankun Qiu Shaobing (Engineering Center for Traditional Chinese Medicine Modernization, East China University of science and technology, Shanghai 200237,China);Advances in the Extraction,Isolation,Purification and Assay Methods of Polysaccharides in Chinese Traditional Medicine[J];Mechanical and Electrical Information;2012-14
5 LIU Zhao-Hui,MU Wei-Dong,WANG Hong,HAN Li-qin(Jilin Medical College,Jilin City,Jilin Province,132013,China);Abstraction of the polyose from radices puerarire,measurement of contents and analysis of removal of oxygen free radicals[J];Journal of Jilin Medical College;2009-01
6 ZHOU Zheng-hui1,2,JIAO Lian-qing1,YU Min1,DAI Wan-run3,ZHAO Cheng-ai2 1.Jilin Academy of Traditional Chinese Medicine,Changchun 130012,China;2 College of Resources and Environment,Jilin Agricultural University,Changchun 130118,China;3.China Pharmaceutical University,Nanjing 210009,China;Studies on Preparation and Structure of Homogeneous Polysaccharides P_Ⅰ,P_Ⅱ from Rhizome of Physalis alkekengi var. franchetii[J];Journal of Jilin Agricultural University;2012-03
7 XU Yu-juan,FAN Su-su,QI Wen-jing,YIN Xiang-tian,REN Hong-mei,LIU Zhen-yu(College of Plant Protection,Shandong Agricultural University,Taian 271018,China);THE INHIBITION EFFECT OF POLYSACCHARIDES OF XANTHIUM SIBIRICUM ON TOBACCO MOSAIC VIRUS AND THE INFLUENCE ON THE DEFENSE ENZYMES ACTIVITIES OF TOBACCO[J];Journal of Shandong Agricultural University(Natural Science Edition);2010-04
8 Yang Ming-jun1,Wu Jing2,Wang Yong-gang1,Li Zhi-zhong1 1(School of Life Science and Engineering,Lanzhou University of Technology,Lanzhou 730050,China) 2(Lanzhou Jinchuan New Meterials Co.,Ltd.,Jinchang 730000,China);Optimization of Extraction Processing of Polysaccharide from Piteguo by Response Surface Methodology[J];Food and Fermentation Industries;2012-01
9 LI Jian1,ZHANG Zhu-qing1,*,CHEN Hui2(1. College of Chemical Engineering,Changchun University of Technology,Changchun 130012,China;2. School of Sciences,Zhejiang Sci-Tech University,Hangzhou 310018,China);Analysis of Flammalina velutipes polysaccharide by gas chromatography and infrared spectra[J];Science and Technology of Food Industry;2010-09
10 GUO Xiao-peng1,TIAN Cheng-rui1,GAO Chun-yan1,MENG You-wu2 (1. College of Food Engineering and Nutrition Science,Shaanxi Normal University,Xi’an 710062,China; 2. Food Limited Liability Company of Shandan Jun Ma Chang,Zhangye 734104,China);Study on removal process of proteins from crude Sphallerocarpus gracilis polysaccharides and its scavenging capability to nitrite[J];Science and Technology of Food Industry;2011-02
Chinese Journal Full-text Database 6 Hits
1 SONG Qun-liang1,ZHANG Ping1,2,SUN Xu-qun2,LI Shao-ping2,HUANG Zhao-gaog2,XIA Quan2(1.Department of Pharmacy,Anhui Medical University,2.The First Affiliated Hospital of Anhui Medical University,Hefei 230032);Determination of polysaccharide in Armillariella tabescens by phenol-sulfuric acid colorimetry[J];Anhui Medical and Pharmaceutical Journal;2007-06
2 QU Chun-xiang, SHEN Song-dong, WANG Xue-feng, CUI Yong-hua, SONG Wei-ping (School of Life Sciences, Suzhou Univ. , Suzhou 215006, China);Method research of measuring soluble protein contents of plant rough extraction using Coomassie Brilliant Blue[J];Journal of Suzhou University(Natural Science Edition);2006-02
3 Tong Hong-li Tian Ya-ping Wang De-qing Deng Xin-xin Dong Zhen-nan Department of Clinical Biochemistry General Hospital of Chinese PLA Beijing 100853 China;Effect of astragalus polysaccharide in regulating blood lipid of rats with hyperlipidemia[J];Chinese Journal of Clinical Rehabilitation;2006-11
4 GE Bin,XU Ai-xia,YANG She-hua(Department of Pharmaceutics, People's Hospital of Gansu Province, Gansu Lanzhou 730000, China);Study on the effect and its mechanism of action of anti-senility of PRASH[J];Chinese Journal of Hospital Pharmacy;2004-10
5 XU Du-juan,CHEN Min-zhu(Institute of Clinical Pharmacology,Anhui Medical University,Anhui Hefei 230032,China);Antitumor activity of APS and its mechanism of action[J];Chinese Journal of Hospital Pharmacy;2005-10
6 Zheng Yi,Gao Yuhong,Wei Guodian,Zhao Zhenhua,Wang Pengxiao(Shandong Province Shengli Oil Field Post Doctor Scientific ResearchWorkstation,Dongying 257055,Shandong,China);Effect of Apes on the Delayed Neuronal Death in the Hippocampus Following Cerebral Ischemia and Reperfusion in Rats[J];Chinese Archives of Traditional Chinese Medicine;2006-07
Chinese Journal Full-text Database 10 Hits
1 QU Xin-hua(Experimental Middle School of Hengyang in Hunan,Hengyang,Hunan 421001);Extraction and Separation Technique of Essential Oil from Plants[J];Journal of Anhui Agricultural Sciences;2007-32
2 ZHAO Dong-wu et al (Zhengzhou Academy of Landscape Planning and Design,Zhengzhou,Henan 450006);Study on the Germplasm Resources Exploitation and Utilization of Yulania Spach from Henan[J];Journal of Anhui Agricultural Sciences;2008-22
3 SUN Jun et al (Forestry Bureau of Nanzhao County in Henan Province,Nanzhao,Henan 474650);Study on the Variety Resources and Classification System of Yulania biondii[J];Journal of Anhui Agricultural Sciences;2008-22
4 LU Xiao-kang et al (Gansu Agricultural University,Lanzhou,Gansu 730000);Study on the Antibacterial Activity of Alfalfa Extract[J];Journal of Anhui Agricultural Sciences;2008-31
5 LI Jian et al(College of Enviroment and Chemical Engineering,Yanshan University,Qinhuangdao,Hebei 610400);Study on Antitumor Activity and Immunomodulating Effect of Polysaccharides Isolated from Solanum nigrum Linne[J];Journal of Anhui Agricultural Sciences;2008-33
6 ZHANG Jian-guo et al(Southwest University of Science and Technology,College of Life Science and Engineering,Mianyang,Sichuan 601010);Optimal Extraction of the Polysaccharide from Red Mushroom[J];Journal of Anhui Agricultural Sciences;2009-03
7 XIA Cui et al(College of Life Sciences and Engineering,Southwest University of Science and Technology,Mianyang,Sichuan 621010);Study on Decoloring Process for Prunella vulgaris Polysaccharide[J];Journal of Anhui Agricultural Sciences;2009-06
8 LIANG Ge et al(Institute of Veterinary Drug,Sichuan Academy of Animal Science,Chengdu,Sichuan 610066);Extraction and Content Measurement of Polysaccharide in Radix cyathulae[J];Journal of Anhui Agricultural Sciences;2009-14
9 LIANG Xiao-hua et al (Department of Chemistry and Life Science,Chuxiong Normal University,Chuxiong,Yunnan 675000);Extraction and Analysis of Total Flavonoids from Alternanthera philoxeroides[J];Journal of Anhui Agricultural Sciences;2010-09
10 LU Xin-yan et al (Zhejiang Forestry University,Lin'an,Zhejiang 311300);Study on the Antioxidant Activity of Polysaccharides Extracted with Alkaline from Lentius edodes by DPPH Assay[J];Journal of Anhui Agricultural Sciences;2010-18
China Proceedings of conference Full-text Database 10 Hits
1 ZHUANG Jin-qiu1,2,MEI Jian-guo1,2,SHEN Zhi-qiang1(1.Shandong Binzhou Animal Science & Veterinary Medicine Academy,Binzhou 256600,China;2.College of Animal Science and Veterinary Science,Jilin University,Changchun 130062,China);BIOLOGICAL FUNCTION OF ASTAGALUS POLYSACCHARIDE AND ITS PROGRESS IN STUDY AND APPLICATION IN SWINE PRODUCTION[A];[C];2012
2 TANG Yao-hua TANG Bo-yong TANG Jin The First Affiliated Hospital of Nanhua University Heng Yang (421001);New concept of anti-cancer treatment-------A~(++) strategy therapy:Chemotherapy combines anti-angiogenic agent and traditional Chinese medicine(TCM) for cancer treatment[A];[C];2011
3 Zhang Zhi-yuan~1,Liu Zhi-hong~(*1),An Xiao-dan~1,Zhu Ling-qin~1,Liu He-rong~1,Zhang Yong1,Wang Guan-jun~2 (1.Ningxia medical college Department of Occupational and Environmental Health,Yinchuan,Ningxia, 750004,china;2.Centers for disease control and prevention of Yinchuan,Yinchuan,Ningxia,750004,china);Study on the damage of Beryllium sulfate and protective effects of Lycium Barbarum Polysaccharides on human embryonic lung Fibroblasts[A];[C];2011
4 LU Wan1,2,QU Zhong-yuan1,2,ZOU Xiang1,2,Yan Qin-qin1,2,JI Yu-bin1,2(1.Engineering Research Center of natural antineoplastic drugs,Ministry of Education;2.Center of Research and Development on Life Sciences and Environmental Sciences,Harbin University of Commerce,Harbin 150076);Advances Research of Juglone[A];[C];2008
5 Dong Rui,Qiu Nongxue,Jin Xiaohui (College of Food Engineering and Nutrition Science,Shaanxi Normal University,Xi'an 710062,China);Comparative Study on Methods of Extraction and Deproteinization of Crude Polysaccharides in Flatstem Milkvetch Seed[A];Chinese Society of Agricultural Engineering[C];2009
6 DONG Yan,SHI Xin-yuan,QIAO Yan-jiang~* (Beijing University of Traditional Chinese Medicine,Beijing 100102,China);Determination of weight average molecular weight of Artemisia halodendron polysaccharide S1 by HPGFC method[A];[C];2008
7 ;Composition analysis and anticoagulant activity study in vitro on mixture of peptide from the pepsin enzymolysis liquid of centipede[A];[C];2009
8 Fu Juan 1,2,Yang Shihai 1,Huang Linfang 2 Chen shilin 2 1.College of Chinese Herbal Medicine,Jinlin Agricultural University,Changchun 130118,China 2.Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences & Peking Union Medical College,Beijing 100193,China;Advances in studies on quality evaluation of Astragalus membranaceus[A];[C];2012
9 HUANG Minyan~(1,2),PAN Linmei~1,FU Tingming~1, PAN Yonglan~1,GUO Liwei~(1*) (1.Key Laboratory of Separation Engineering for Chinese Medicine Compound, Nanjing University of Traditional Chinese Medicine,Nanjing 210029,China; 2.Department of Pharmacy,The First People's Hospital of Nantong,Nantong 226001,China);Study on the mechanism and prevention of membrane fouling during refining Sanliangban liquor[A];[C];2012
10 ZHANG Lianjun~(1,2),GUO Liwei~(1*),FU Tingming~1 (1.Key Laboratory of Separation Engineering for Chinese Medicine Compound,Nanjing University of Chinese Medicine,Nanjing,Jiangsu 210029;2.Jiangsu Provincial Rongjun Hospital,Wuxi,Jiangsu 214035));The study of pretreatment on the microfiltration of HuangLianJieDu Decoction with ceramic membranes[A];[C];2012
Chinese Journal Full-text Database 10 Hits
1 WANG Xue-mei1,GAO Su-lian1,LIU Ming2(1.College of Chemistry and Chemical Engineering, Anhui University, Hefei230039, China;2.Affiliated Hospital, Anhui Medical University, Hefei230022, China);The application of abelmoschus manihot in cosmatics-anti-acne beauty toner[J];Journal of Anhui University(Natural Sciences);2003-01
2 WANG Xue-mei,HONG Feng,ZHANG Jian (School of Chemistry and Chemical Engineering,Anhui University,Hefei 230039,China);Extraction of natural sunscreen from the blossom of abelmoschus manihot by supersonic method[J];Journal of Anhui University(Natural Sciences);2005-04
3 Chen Yan et al (School of Life Science,Anhui University ,Hefei 230039);Purification and Composition Analysis of a Polysaccharide from Seaweed[J];Journal of Anhui Agricultural Sciences;2003-05
4 XIN Yan-wei(Shanxi Agricultural University,Taigu,Shanxi 030801);Exploiture and Application of Elaeagnus angustifolia L.[J];Journal of Anhui Agricultural Sciences;2007-02
5 ZHANG Rong-xian et al(College of Life Sciences,Sichuan University,Chengdu,Sichuan,610064);Effects of Extracts from Prinsepia utilis Royle on Antimicrobial Activity[J];Journal of Anhui Agricultural Sciences;2007-02
6 LI Zhu-ying et al(Yuxi Agricultural Institute,Yuxi,Yunnan 653100);Formalization Cultural Technique of Schizophyllum ocmmuner Fr.[J];Journal of Anhui Agricultural Sciences;2007-03
7 JIANG Ning et al(College of Biological Science and Technology,Hubei Institute for Nationalities,Enshi,Hubei 445000);Study on the Optimization of Liquid Culture Medium in Flammuling velutipes Cart.Sing[J];Journal of Anhui Agricultural Sciences;2007-21
8 HUANG Sheng-quan et al(College of Light Industry and Food Sciences,South China University of Technology,Guangzhou,Guangdong 510640);Study on Extraction Technology of Water-soluble Polysaccharide from Ganoderma lucidum by Ultrasonic Assistant Method[J];Journal of Anhui Agricultural Sciences;2009-23
9 ZHOU Hong-li et al(School of Pharmaceutical Sciences,Jilin University,Changchun,Jilin 130021);Study on Extraction and Deproteinization of Polysacharide in the Corn Silk[J];Journal of Anhui Agricultural Sciences;2009-34
10 ZHOU Hong-li et al (School of Chemical and Pharmaceutical Engineering,Jilin Institute of Chemical Technology,Jilin,Jilin 132022);Study on the Deproteinization of Corn Stigma Polysaccharide by Sevag Method[J];Journal of Anhui Agricultural Sciences;2010-28
【Secondary References】
Chinese Journal Full-text Database 10 Hits
1 NIE Guang-jun1,2,YUE Wen-jin1,ZHENG Yi1,ZHU Xiao-jun2(1.Coll.of Bioch.Engn.,Anhui Polytechnic University,Wuhu 241000,China;2.Wuhu Tiyilvbao Biological Technolgoy Lmt.Co.,Wuhu 241000,China);Extraction of polysaccharides from Lemna minor L. by Stacking method[J];Journal of Anhui University of Technology and Science(Natural Science);2010-04
2 XIAO Huai-qiu,LI Yu-zhen,LIU Jun(Applied Chemistry Department of Hunan Chemical Industry and Vocational Technology College,Zhuzhou 412004,China);Separation and Purification of Polysaccharide from Lygodium japonicum Decoction and Its Spatial Structure[J];Amino Acids & Biotic Resources;2011-04
3 LIU Han-zhen1,XIAO Xin1,MAO Bin-bin1,MA Shi-tang1,DU Jin-dan1,WANG Wen-ge2(1.College of Food and Drug,Anhui Science and Technology University,Fengyang 233100,China; 2.Yaoluoping Nature Reserve Management Committee,Yuexi 246600,China);Optimization Craft of Flavanone Class Ingredient from Dendranthema Morifolium ‘Chuju’[J];Journal of Anhui Science and Technology University;2011-05
4 LUO Xiang-dan1,YAO Gang2,ZHANG Tie-hua1(1.Department of Deeply Processing of Agricultural Products,College of Quartermaster Technology, Jilin University,Changchun 130062,China;2.Department of Neurology, Second Hospital,Jilin University,Changchun 130041,China);Effects of three kinds of fungi polysaccharides on fermentation characteristics of lactobacillus acidophilus[J];Journal of Jilin University(Medicine Edition);2011-06
5 ZHANG Shiyuan1 XU Chunyan1 DONG Xiaofang2 TONG Jianming2 ZHANG Qi2 (1. Institute of Animal Science and Technology,Yangzhou University,Yangzhou 225009,China; 2. State Key Laboratory of Animal Nutrition,Institute of Animal Science,Chinese Academy of Agricultural Sciences,Beijing 100193,China);Effects of Water Soluble Alfalfa and Astragalus Polysaccharide on Proliferation of Lymphocyte in Broilers[J];Chinese Journal of Animal Nutrition;2010-03
6 CHEN Hu-hu1,2, SUN Jing3, YANG Jin-ying1, GONG Su-xiao2, ZHANG Tie-jun2 1. Jiangsu Hansoh Pharmaceutical Co., Ltd., Lianyungang 222001, China 2. Tianjin Institute of Pharmaceutical Research, Tianjin 300193, China 3. Tianjin Medical University, Tianjin 300070, China;Determination of monosaccharide compositions in Astragalus mongholicus var. mongholicus polysaccharides by pre-column derivatization-HPLC[J];Drugs & Clinic;2012-05
7 Yang Shaoli,Liu Zhiming*(Key Laboratory of Bio-based Material Science and Technology of Ministry of Education,College of Material Science and Engineering,Northeast Forestry University,Harbin 150040,China);Preparation Technology Optimization Analysis of Bamboo Pulp Nano-cellulose[J];Guangdong Chemical Industry;2012-15
8 ZHOU Jian-guo et al(College of Bio-safety and Technology,Hunan Agricultural University,Changsha,Hunan 410128);Research Progress of Occurrence and Control of Tobacco Mosaic Virus[J];Journal of Anhui Agricultural Sciences;2013-01
9 ZHANG Zhen1,YAN Xiaohui2,MU Shuzhen3,HAO Xiaojiang3*(1.College of Chemical Engineering,Guizhou University,Guiyang,Guizhou 550001;2.College of Forestry,Southwest Forestry University,Kunming,Yunnan 650224;3.Key Laboratory of Chemistry for Natural Products of Chinese Academy of Sciences,Guiyang,Guizhou 550002,China);Isolation of Sempervirine with Its Anti-TMV Activity[J];Guizhou Agricultural Sciences;2013-05
10 ZHENG Li1,HAN Ping1,LIU Tao2,CUI Zhi-song1,GUO Xiu-chun1,LUO Dan2,GAO wei1(1.The First Institute of Oceanography,State Oceanic Administration,Qingdao 266061,China;2.Ocean University of China,Qingdao 266003,China);Fluorescence quantitative detection and extraction process optimization of laminarin from kelp[J];Marine Sciences;2012-05
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 LIU Li-chao,HUANG Hong-lin,LAI Zheng-quan,LIAO Hui-jun(Jiangxi College of Traditional Chinese Medicine,Nanchang 330006,China);Determination of water soluble polysaccharide in fructus gardeniae[J];Anhui Medical and Pharmaceutical Journal;2005-11
2 LU Jianliang LIANG Yuerong AHANG Lingyun (Tea Research Institute, Zhejiang University, Hangzhou, 310029);Modification of coomassic brilliant blue method and application to protein assay of tea liquor[J];Journal of Tea;2002-02
3 ZHANG Hong-ying,SUN Yan-qiu,L Shen [WT5BX](Department of Pathology,Dalian Medical University,Dalian 116027,China) [WT5HZ];Effect of TPN being added Huang Qi upon the immune function of patients with stomach cancer in perinatal surgery[J];JOURNAL OF DALIAN MEDICAL UNIVERSITY;2000-02
4 GAO Ting1, BAI Jia-feng1, LUO Xia2, CHEN Dong-hui2,XU Fei-yi1 CHEN Zhi1, CHEN Yong-zhu1, Sun Qi-ling1(1. School of Life in Sichuan University, Chengdu 610064,China; 2. Sichuan Institute of Chinese Materia Medica, Chengdu 610041,China);The Effects of Armillariella Polysaccharide on the Immune Function of Mice[J];Journal of Sichuan University (Natural Science Edition);2005-02
5 Guo Minliang;Jiang Yongming(Basic Science Division; Jiangsu Agricultural college;Yangzhou 225009;China).;^Effect of Ingredients of Coomassie Brilliant Blue Color-developing Reagent on Protein Assay.[J];PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS;1996-06
6 SHAN Jun-jie, WANG Shu-chun, LIU Di, Hu Zhi-bi Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine;Progress of Chemical and Pharmacological Study of Astragalus Polysaccharide[J];ACTA UNIVERSITATIS TRADITIONIS MEDICALIS SINENSIS PHARMACOLOGIAEQUE SHANGHAI;2000-03
7 Ying Zizhong, Han Zhihong Wuhan Professional Medical College, Wuhan 430016;Effects of Huangqi on Immune Function of Transplanted Tumors of Mice[J];LiShiZhen Medicine and Materia Medica Research;1999-10
8 Yin Jun-qing,Song Shu-zhen,Tian Ya-ping,Wang De-qing,Department of Biochemistry,General Hospital of Chinese PLA,Beijing 100853,China Yin Jun-qing,Technician-in-charge,Department of Biochemistry,General Hospital of Chinese PLA,Beijing 100853,China;Interventional effect of astragalus polysaccharide on blood lipids in healthy people[J];Chinese Journal of Clinical Rehabilitation;2005-31
9 NIE Wei, ZHANG Yong Xiang (Institute of Pharmacology and toxicology,Academy of Military Medical Sciences, Beijing 100850);Progress of the immuno modulating effect of polysaccharides and their mechanisms[J];Chinese Pharmacological Bulletin;1999-06
10 NIU Chao Shi, SUN Jing Wu, BIAN Liu Guan, LUO Qi Zhong (Dept of Neurosurgery, Renji Hospital Affiliated to Shanghai Second Medical University, Shanghai 200001);The growth inhibition and induction of apoptosis in rat C 6 glioma cells by TNF-α[J];CHINESE PHARMACOLOGICAL BULLETIN;2000-02
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