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《Anhui Medical and Pharmaceutical Journal》 2009-06
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Determination of costunolide and dehydrocostuslactone in Radix Auck landiae produced in different areas by HPLC

ZHAO Xiao-ming1,YIN Gao-bin2(1.Rugao People's Hospital;2.The Second Peple's Hospital,Rugao,Jiangsu 226500)  
Aim To establish an HPLC method for determining costunolide and dehydrocostuslactone in Auck landia lappa Decne.Methods The HPLC system consisted of Zorbax SB-C18(4.6 mm×150mm,5 μm) with methanol-water(65∶35) as the mobile phase and the detective wavelength at 225 nm.And the flow rate was 1.0 ml·min-1.Results The costunolide and dehydrocostuslactone equations were AI=14.59C-13.28(=0.999 8) and AⅡ=11.27C-14.27(r=0.9994),respectively.The average recovery of costunolide and dehydrocostuslactone was 101.9 % and 100.4 %,respectively,and RSD was 1.25 % and 0.99 %.Conclusion The content of costunolide and dehydrocostuslactone was different in the Radix Auck landiae.produced in different areas.The method is simple,accurate and can be used for quality control of the Radix Auck landiae.
【Key Words】： HPLC costunolide dehydrocostuslactone
【CateGory Index】： R284
【DOI】： CNKI:SUN:AHYY.0.2009-06-018
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【Citations】
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1 MENG Hui-ning1,LUO Guo-ping2(1.Shanxi University of Chinese Medicine,Xianyang,Shanxi Province,712000;2.Xi'an Medical College,Xi'an,Shanxi Province,710021);Determination of rutin in the leaf of hibiscus mutabilisL. by HPLC[J];Anhui Medical and Pharmaceutical Journal;2007-08
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3 HUANG Li-dan(Anhui Provincial Institute for Food and Drug Control,Hefei 230051);Determination of paeonol in radix etrhizoma cynanchi paniculati[J];Anhui Medical and Pharmaceutical Journal;2008-07
【Co-citations】
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1 PENG Wen-li1,ZHOU Wen-yan2(1.Traditional Chinese Medicine Hospital of Qimen,Qimen 245600,China;2.Hefei Kangcheng Technology Ltd,Hefei 230023,China);Determination of hesperidin contents in yangweishu granules by HPLC[J];Anhui Medical and Pharmaceutical Journal;2008-04
2 TAO Jun-yu,BAO En-quan,QIN Shao-gang,WANG Wei (Chaohu Institute for Drug Control,Chaohu 238000,China);Determination of rutin in the proplis tablets by HPLC[J];Anhui Medical and Pharmaceutical Journal;2008-06
3 DAI Long(Shandong University of Traditional Chinese Medicine,Jinan 250355,China);Determination of hydrochlorothiazide and rutin in zhenjujiangya tablet by HPLC[J];Anhui Medical and Pharmaceutical Journal;2009-05
4 ZHAO Hong,HU Rong-feng(Department of Pharmacy,Anhui College of Traditional Chinese Medicine & Anhui Provincial Key Laboratory for Research and Development of Chinese Medicine,Anhui Hefei 230031,China);Content Determination of Paeonol in Delayed-release Microspheres[J];Journal of Anhui Traditional Chinese Medical College;2009-02
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8 YU Ying (Department of Nephrology,The Third Affiliated Hospital of Anhui Medical University,Hefei 230061,China);Determination of hesperidin in Zhengchaihuyin capsules by HPLC[J];Anhui Medical and Pharmaceutical Journal;2009-12
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