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《Anhui Medical and Pharmaceutical Journal》 2010-07
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Extraction of polysaccharide constituents from chaenomeles speciosa by cellulose enzyme

LIU Zhao-xia1,3,LI Shi-gang2,CHEN Yan2,ZHOU Jie1(1.Hubei Key Laboratory of Natural Products Research and Development,College of Chemistry and Life Sciences,Three Gorges University,Yichang,Hubei 443002,China;2.Medical College,Three Gorges University,Yichang,Hubei 443002,China;3.Tongji School of Pharmacy,Huazhong University of Science and Technology,Wuhan,Hubei 430030,China)  
Aim The phenol-sulfuric acid method was used to determine the content of polysaccharide from chaenomeles speciosa(sweet)Nakai.Methods Copared with traiditionl metheds,chaenomeles speciosa which dealt with cellulose enzyme.Result And the precision,repeatability,stabability etc.of this method were good.The standard curver was Y=0.008 8X-0.010 9(r=0.996 2).Conclusions Besides,after dealt with cellulose enzyme the yield of polysaccharide increased by 76.67%from 5.23%to 9.24%.
【Fund】: 三峡大学青年科学基金项目(NoKJ2008A001) 三峡大学科研基金(No0620070105)
【CateGory Index】: R284
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