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《Anhui Medical and Pharmaceutical Journal》 2012-03
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Determination of gardenoside in jinhuaxiaocuo particle by HPLC

CHEN Gen-de 1,WU De-ling 2,XING Rong 1(1.Department of Pharmacy,First Affiliated Hospital of Bengbu Medical College,Bengbu 233000,China; 2.School of Pharmacy,Anhui College of Traditional Chinese Medicine,Hefei 230031,China)  
Objective To establish an HPLC method to determine gardenoside in jinhua xiaocuo particles.Methods A Shim-pack C18(4.6 mm ×250 mm,5 μm) was used with the mobile phase of acetonitrile-water(15∶ 85).The detection wavelength was 238 nm and the temperature was 40℃.The flow rate was 1.0 ml·min-1.Results Gardenoside sample size in 0.052 4~0.524 0 μg within the range of peak area showed good linear relationship,and the average recovery was 98.56%.RSD was 2.06%(n=6).Conclusion The determination method is simple,stable,precise,and reproducible.
【CateGory Index】: R286
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