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DPPH radical scavenging effect of poplar bud extracts

LIU Chang-long,LEI Hui,ZHAO Qi-xiu,et al(School of Pharmacy,Nanjing University of Chinese Medicine,Nanjing Jiangsu 210023,China)  
Objective To make preliminary extraction and separation of poplar buds,and to determination its total flavonoids and antioxidant activity.Methods By ultrasound we extracted the active ingredients in the poplar buds.Ascorbic acid,tocopherol and rutin as reference,we measured free radical scavenging effect by DPPH method.Results Ultrasonic assisted extraction of poplar buds contained 14.4% of total flavonoids,whose radical scavenging capacity was higher than rutin and tocopherol when its concentration reached 0.05 g.L-1.With increased concentration the scavenging capacity was slightly lower than tocopherol but higher than rutin.And the capacity increased with the increase of concentration.Conclusion Poplar bud extracts had high flavonoid content and strong antioxidant activity.
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