ANTI-VIRAL EFFECTS OF TOTAL POLYSACCHARIDES OF ASTRAGALUS MEMBRANACEUS ON HERPES SIMPLEX VIRUS TYPE-2
WANG Zhi jie,HUANG Tie niu,LIU Yan wen,YANG Zhan qiu,XIAO Hong (Virology Institute, Medical College, Wuhan University, Hubei Wuhan 430071, China)
Objective:To study the anti viral effects of total polysaccharides of Astragalus membranaceus (PSA). Method: Acyclovir (ACV) was used as positive control drug. The anti viral effects of PSA on 333 strain of herpes simplex virus type 2 (HSV 2 333 strain) were observed by using plaque reduction assay and observing the inhibitory effect of PSA on cellular pathological changes induced by herpes. Result: In Hep 2 cell system, the median effective dose (ED 50 ) of direct virucidal effect, inhibiting multiplication effect, interrupting infection effect of ACV on HSV 2 333 strain was 16.45,18.62,10.85 μg/ml, respectively;The corresponding figures of PSA were 6.04,5.43,7.50 μg/ml, respectively. Conclusion: The treatment index of PSA for HSV 2 333 strain is 3.7, 2.7, 3.3 fold as that of ACV, respectively. It is worthy of exploiting.