EFFECT OF ELECTRO-ACUPUNCTURING DU CHANNEL ON NEUROCYTE APOPTOSIS IN ISCHEMIC CEREBRAL INJURY RATS
LUO Zhong-da Tutor: Professor LAI Xin-sheng,Doctor XU Neng-gui (Futian Hospital of Traditional Chinese Medicine of Shenzhen City,Guangdong Shenzhen 518034,China)
Objective:To research the molecular mechanism of electro-acupuncture (EA) in the treatment of ischemic cerebral vascular diseases. Method: By using in situ labeling technology,the effects of electro-acupuncturing Dazhui (Du 14),Baihui (Du 20) on ischemic cerebral injury were observed. 10 hours after focal cerebral ischemia induced by coagulation of rat middle cerebral artery, by using terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling(TUNEL), the effects of EA on neurocyte apoptosis were observed. Result: The TUNEL staining positive cells of ischemic region of cortical layer in EA group were significantly decreased as compared with those in model group (P0.01). Conclusion: Acupuncture can restrain the neurocyte apoptosis induced by ischemic cerebral injury.