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《Journal of Anhui Traditional Chinese Medical College》 2008-02
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Study on Baicalin Induced Apoptosis of Human Breast Cancer Cell Line MDA-MB-231

ZHU Guo-qi,TANG LI-jian,WANG Lei,SU Jing-jing,WANG Ning,PENG Dai-yin,LI Qing-lin(Anhui College of Traditional Chinese Medicine & Anhui Key Laboratory of Modern Chinese Materia Medica,Anhui Hefei 230038,China)  
Objective: To study the baicalin induced apoptosis of human breast cancer cell line MDA-MB-231(ER-).Methods: The inhibitory effect of different concentration of baicalin on the MDA-MB-231 cells was measured by MTT method.Transmission electron microscope was employed to observe the apoptosis of the cells.Cell cycle was analysed by flow cytometry.The mRNA level expression of bcl-2 and bax was determined by RT-PCR.Results: The proliferation of MDA-MB-231 cells was inhibited by baicalin in dose-and time-dependent manner and the IC50 was 151 μmol/L.The apoptotic rate of the baicalin-treated MDA-MB-231 cells increased significantly at 48 h.Flow cytometer analysis also revealed that most of the baicalin-treated MDA-MB-231 cells were arrested in the G2/M phase.Typically apoptotic characteristics such as condensed chromatin and apoptotic bodies were observed after being treated with baicalin for 48 h.The results of RT-PCR showed that the expression of bax was up-regulated;meanwhile,the expression of bcl-2 was down-regulated.Conclusion: Baicalin could inhibit the proliferation of MDA-MB-231 cells through apoptosis by regulating the expression of bcl-2,bax and intervening the process of the cell cycle.
【Fund】: 安徽省人才开发基金项目(2005ZD036)
【CateGory Index】: R285;R737.9
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1 ZHAO Liang-cai~(1),TANG Li-jian~(1),LI Qing-lin~(1),LIU Jing-gen~(2)(1.Anhui College of Traditional Chinese Medicine,Anhui Key Laboratory of Modernized Chinese Material Medical,Hefei 230031,China;2.Shanghai Institute of Materia Medica,Shanghai 201203,China);Advancl on phytoestrogen intervening breast cancer[J];China Journal of Chinese Materia Medica;2006-12
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