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《Journal of Anhui Traditional Chinese Medical College》 2008-04
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Effects of Buyang Huanwu Tang on Ca~(2+) Message of Global Cerebral Cortical Neurons in Rats with Ischemia and Reperfusion at Different Time Points

GUAN Li,YAN Fu-man,ZHANG Xiao-dong,SU Wen,LI Xiao-ying,ZHOU Le-quan,LUO Rong-jing(Guangzhou University of Traditional Chinese Medicine,Guangdong Guangzhou 510405,China)  
Objective: To apply the patch clamp and the ion-image analytical technique to simultaneously study the effects of Buyang Huanwu Tang on the Ca2+ L-channels and the intracellular Ca2+ concentrations of the ischemic rats′ cerebral cortical neurons,intensively discuss the mechanism that the abnormity of the neuronal Ca2+ message joins in the ischemic neuronal injury and the molecular mechanism of cerebral anti-ischemia with Buyang Huanwu Tang,provide the theoretical basis for the prevention and treatment of the ischemic encephalopathy with this prescription,and explore new ideas and methods for the research on the complex prescription.Methods: Consulted the modified Pulsinelli′s tetra-vessel occlusion to prepare the global cerebral ischemic rats.The rat cerebral cortical cell was acquired in the acute separation method.And the Ca2+ concentration of a single neuron was tested by the Ca2+ image-forming system.Records and analysis of the single-channel electricity: the recording single-channel current was amplified by the EPC-9 patch-clamping amplifier,and collected into the computer by Pulsefit+Pulse.It was tested by the analytical software TAC.Fifty percent of the electrical amplitude at some recording voltage was considered as the transition to judge the channel′s opening and closing.The acquired data was statistically processed by the software TACFIT.Results: The preintervention by Buyang Huanwu Tang had the definite effect on dropping the Ca2+ concentration of the global cerebral cortical neuron of rats with ischemia and reperfusion.Especially,the effect was more significant when the Ca2+ concentration reached the peaks(at 2 h and 24 h after reperfusion).The preintervention by Buyang Huanwu Tang had the definite effect on dropping the opening probability of the Ca2+ L-channels of the rats′ cerebral cortical neurons after reperfusion.Especially,the effect on drop was more significant when the probability reached the peak(at 2 h after reperfusion).Conclusion: It was the main mechanisms of the cerebral anti-ischemia with Buyang Huanwu Tang to drop the Ca2+ concentration,the average opening time and the opening probability of Ca2+ L-channels of the global cerebral cortical neurons of the rats with ischemia and reperfusion at different time points.The preintervention by Buyang Huanwu Tang was of significance to the prevention and treatment of the ischemic neuronal injury.
【CateGory Index】: R285.5
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