Content Determination of Paeonol in Delayed-release Microspheres

ZHAO Hong,HU Rong-feng(Department of Pharmacy,Anhui College of Traditional Chinese Medicine & Anhui Provincial Key Laboratory for Research and Development of Chinese Medicine,Anhui Hefei 230031,China)  

Objective:To establish a high performance liquid chromatography(HPLC) method for content determination of paeonol in delayed-release microspheres.Methods:Chromatographic experiments were performed on Kromasil C18 column(40 mm×250 mm,5 μm).The mobile phase was composed of methanol-water(52∶48).The detection wavelength was 274 nm.The flow rate was 1.0 ml/min.The column temperature was 30℃.Results:The linear range was 0.3124～7.810 0 μg/ml(r=0.999 6).The average recovery was 97.11% with RSD=1.56%(n=3).Conclusion:This method is simple,accurate,and suitable for the quality control of delayed-release paeonol microspheres.

【Key Words】： Paeonol Content determination High performance liquid chromatography
【Fund】： 安徽省高等学校省级自然科学研究项目(KJ2009A112)
【CateGory Index】： R286.0
【DOI】： CNKI:SUN:AHZY.0.2009-02-021

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