EFFECTS OF BIFENDATI AND THREE CHINESE TRADITIONAL MEDICINAL HERBS ON AFLATOXIN B1( AFB1)-INDUCED HEPATOCARCINOGENESIS IN RATS
YAN Rui-qi CHEN Zhi-ying QIN Guo-zhong(Department of pathology, Guangxi Cancer Institute, Nanning)
Bifendati and three Chinese Traditional Medicinal Herbs:Angelica sinensis (Oliv)Diels (AD).Fructus schisandra chinensis (FC) and Smilax glabra Roxb (SR)were tested for their effects on AFB1-induced r-glutamyltranspeptidase ( r-GT) posi t i ve hepatocel lular foci in rats. The rats Were given 12 i.p. doses of initiator AFB1 (280μg/kg, dose, 6 times per week) in two successive weeks. Two weeks after initiation, the rats were submitted to a promotion program, i.e., a two-week's feeding of 0.015% acetylaminofluorene-containing diet plus a two -third hepatectomy at the middle of the feeding period. Ten days after partial Repatecto my, the rats were sacrificed and the livers were processed to r-GT staining. The tested herbs were extracted repeatedly with 95% alcohol and the concertrated extracts were mixed with the basal diet. The administration of drug-containing diet was started 10 days before AFB1 and finished 3 days after the di scont innat ion of AFB1, The livers of the rats taken Bifendati, AD, and FC showed significantly less and smaller r-GT foci, indicating that these tested substances with therapentic effect for hepatitis are effective in inhibiting AFB1-induced hepatocarcinogenesis.