THE USE OF MTT ASSAY FOR THE RAPID TESTING OF CYTOTOXICITY OF DRUGS AGAINST NON-SOLIDTUMOR CELLS
LI Deng-hua TIAN Zhi-gang ZHANG JieZHANG Jian-hua CU Zheng-yan (Department of Immunology, Shandong Academy of Medical Sciences, Jinan 250001)
The MTT assay method was established and used for the rapid testing of cytotoxicity of different drugs(Mitomycin-C, 5-Fluorouracil, Adriamycin and cytos-sine arabinoside) against different non-solid tumor cell lines (YAC-1, EL-4, MLA144and bcmg cells). The result showed that there was a linear relationship between viable cell numbers and formazan production (expressed by optical density). This could show not only the suppressing patterns of one cell line to different chemotherapentic agents, but also different sensitivity of tumor cells to each drug. The results indicated that the MTT assay might offer a practical, economical, rapid, simple and very efficient method of assessing cytotoxicity of drugs in non-solid tumors.