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《Chinese Journal of Cancer》 1991-03
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THE USE OF MTT ASSAY FOR THE RAPID TESTING OF CYTOTOXICITY OF DRUGS AGAINST NON-SOLIDTUMOR CELLS

LI Deng-hua TIAN Zhi-gang ZHANG JieZHANG Jian-hua CU Zheng-yan (Department of Immunology, Shandong Academy of Medical Sciences, Jinan 250001)  
The MTT assay method was established and used for the rapid testing of cytotoxicity of different drugs(Mitomycin-C, 5-Fluorouracil, Adriamycin and cytos-sine arabinoside) against different non-solid tumor cell lines (YAC-1, EL-4, MLA144and bcmg cells). The result showed that there was a linear relationship between viable cell numbers and formazan production (expressed by optical density). This could show not only the suppressing patterns of one cell line to different chemotherapentic agents, but also different sensitivity of tumor cells to each drug. The results indicated that the MTT assay might offer a practical, economical, rapid, simple and very efficient method of assessing cytotoxicity of drugs in non-solid tumors.
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【References】
Chinese Journal Full-text Database 10 Hits
1 Yan Maoxiang ; Chen Zhiyun ; Cao Junmin , et al(The Affiliated Hospital to Zhejiang College of TCM, Hangzhou 310006);The Influence of Wei Er Kang on Immunologic Function in Rats[J];JOURNAL OF ZHEJIANG COLLEGE OF TCM;1998-03
2 HOU Wei,XIAO Hong,MAO Lin,XIAO Chun-hui,CHENG Li,XIONG Zeng-hui,YANG Ji-jiang,YANG Zhan-qiu (Institute of Virology, School of Medicine, Wuhan University, Wuhan 430071, China;Medicine Industry Institute of Hubei, Wuhan 430000, China; Virus Research Center, Zhongshan Medical College, Taiwan);Observation for antiviral activity of Penciclovir in vitro[J];West China Journal of Pharmaceutical Sciences;2004-02
3 liu Yufeng,Xu Lizhi,Zhang Chengwu, Wei Haiyan, Li Heng (Medical School of Nanjing University, Nanjing 210093);THE INHIBITION OF PHYCICYANIN FROM PORPHYRA HAITANENSIS ON THE GROWTH OF HUMAN LEUKEMIA HL-60 CELLS[J];CHINESE JOURNAL OF MARINE DRUGS;2000-01
4 Tao Qian;He Rong-geng;Zhou Xiao-jian(Department of Oral &Maxillofacial Surgery,9th Hospital,Shanghai Second Medical University,Shanghai 200021);THE EXPERIMENT STUDY OF STREPTOCOCCAL PREPARATION 722 IN THE TREATMENT OF B16 MELANOMA[J];CHINESE JOURNAL OF ORAL & MAXILLOFACIAL SURGERY;1996-02
5 Li Xiaohong Wu Juan Lin Ling Gu Haiming Tao Yanmei Zhang Zuxuan (School of Medicine,Nanjing University,210093,Nanjing,PRC);INHIBITING EFFECT OF IM 94 ON NEOPLASMA CELLS[J];JOURNAL OF NAIJING UNIVERSITY (NATURAL SCIENCES);1997-02
6 Cai Jinglong;Li Denghua;Zhang Jianhua(Dept.of General Surgery, the Affiliated Hospital of Shandong Medical University,Shandong Academy of Medical Sciences);STUDY ON THE USE OF MTT ASSAY FOR THE CHEMOSENSITIVITY TESTS OF STEREOTUMOUR CELLS[J];ACTA ACADEMIAE MEDICINAE SHANDONG;1994-04
7 Fang Mingzhu, Zhang Weiming,He Nengshu,et al The Experiment Center of Tianjin Medical University;Chemosensitivity Testing of Lung Cancer Cells Using the MTT Assay[J];Tianjing Medical Journal;1996-01
8 Du Gangjun, Lin Haihong, Yang Yiming 1, Zhang Shui Lan 1(Department of Pharmacology, Pharmaceutical college of HeNan University, KaiFeng, 475001; 1Department of oncology , Dong Jing Hospital of HeNan University , Kai Feng 475;Study on antitumor and antimetastasis effect of alcohol extract from roots of rubus crategefolius bge(ERC)[J];Chinese Journal of Modern Applied Pharmacy;2002-05
9 ZL Xu, JH Guo, ST Song, MJ Li and DZ Wu (Cancer Research Center, North Taiping Road Hospital, Beijing 100039);THE EFFECT OF ZINC GLYCYRRHIZATE ON TOXICITY AND ANTICANCER ACTIVITY OF CISPLATIN IN MICE[J];Acta Pharmaceutica Sinica;1993-08
10 ZHOU Jian-Jun~*, YUE Xiu-Fang, HAN Jia-Xian, YANG Wei-Yi (Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 200031);IMPROVED MTT ASSAY FOR ACTIVITY OF ANTITUMOR AGENTS[J];Chinese Journal of Pharmaceuticals;1993-10
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 Huang Zhenxue, et al Department of Hygiene;STUDY OF RELATIONSHIP BETWEEN ZINC AND IMMUNE FUNCTIONS[J];;1988-03
2 ~1Shen Shengrong;~1Yang Xianqiang(~1Department of Tea Science,Zhejiang Agricultural University,Hangzhou)~2Zhao Baolu;~2Xin Wenjuan(~2Institute of Biophysics,Chinese Academy of Sciences,Beijing);Scavenging Effects of Tea Polyphenol Compound and(-)-EGCG on Oxygen Free Radicals[J];Journal of Tea Science;1992-01
3 Shen Shengrong~1;Yang Xianqiang~1;Zhao Baolu~2;Xin Weniuan~2(~1Department of Tea Science,Zhejiang Agricultural University,Hangzhou310029)(~2Institute of Biophysics,Chinese Academy of Siences,Beijing100080);Radical Mechanism of Prooxidant Effect on Tea Polyphenols(TP)in vitro[J];Journal of Tea Science;1992-02
4 Shen Shengrong~1;Yang Xianqiang~1;Yang Fajun~2;Zhao Baolu~2;Xin Wenjuan~2;Yukihiko Hara~3(1 Institute of Tea Science,Zhejiang Agricultural University,Hangzhou310029)(2 Institute of Biophysics,Chinese Academy of Sciences,Beijing100101)(3 Food Research Laboratories,Mitsui Norin Co.,Shizuoka 426,Japan);Coordinatingly Synergic Effect of Catechins During Their Antioxidation[J];Journal of Tea Science;1993-02
5 Xia Tao Key Laboratory of Tea Biotechnology of Agricultural Ministry,Anhui Agricultural University, Hefei 230036;Study on the Mechanism of Black Tea Pigments Formation[J];JOURNAL OF TEA SCIENCE;1999-02
6 Xiao Weixiang 1,Wan Xiaochun 1,Hu Yaowu 2,Zhong Jin 1,Xiao Hui 1 1 Anhui Agricultural University Hefei 230036 2 University of Science and Technology of China, Hefei 230036;Study on the in vitro Oxidation Product of Tea Catechines[J];JOURNAL OF TEA SCIENCE;1999-02
7 Wang Yuefei 1,Gao Yonggui 1,Zhou Yaqin 2,Yang Xianqiang 1,Xu Chunyin 1 1 Tea Science Department of Zhejiang University, Hangzhou 310029;2 Physics Department of Beijing University, Beijing 100871;Interaction between Tea and DNA and Effects of Vitamin C and Caffeine[J];JOURNAL OF TEA SCIENCE;1999-02
8 Shen Shengrong 1, Zhao yufang 1, Yang Xianqiang 1, Yao Side 2 (1 Department of Tea Science Zhejiang Agricultural University, Hangzhou 310029; 2 Laboratory of Radiation Chemistry,Institute of Nuclear Research,Academia Sinica,Sha;Kinetics of the Reaction Between Catechins and Hydroxyl Radicals[J];JOURNAL OF TEA SCIENCE;1997-S1
9 Shen Shengrong 1, Zhao Yufang 1, Zhao Baolu 2 (1 Zhejiang Agricultural University, Hangzhou 310029; 2 Institute of Biophysics,Academica Sinica,Beijing 100101);Inhibiting Effect of Catechins to Free Radicals at the Present of Iron Ion[J];JOURNAL OF TEA SCIENCE;1997-S1
10 Zhou Caiqiong,Liu Xianjun,Zhou Weihua,Wang Guangchi (Food Science College,Southwest Agricultural University,Chongqing 400716);The Way of Reducing Nitrite Content in Pickled Lettuce with Tea Polyphenols[J];JOURNAL OF TEA SCIENCE;1998-02
【Secondary References】
Chinese Journal Full-text Database 10 Hits
1 SU Zhong-liang et al (Department of Biology,College of Chemical Industry,Qingdao University of Science and Technology,Qingdao,Shandong 266042);Research Progress of the Activity and Application of Phycobiliprotein[J];Journal of Anhui Agricultural Sciences;2008-30
2 QING Chen,PU Shao ping,LI Ma lin,et al The Key Laboratory of Natural Drugs and Pharmocology in Yunnan Province,Kunming 650031,P R China;Inhibitory effect of JM216 on human ovarian tumor HO-8910[J];CHINESE JOURNAL OF CANCER;2000-04
3 ZHANG Yan, JIANG Ji kai*, LIU Xiao shan, et al Department of Clinical Biochemistry, Chongqing University of Medical Sciences, Chongqing 400016, P R China;Differentiation and apoptosis in K562 erythroleukemia cells induced by matrine[J];CHINESE JOURNAL OF CANCER;2000-08
4 MA Zhong cai, HE Geng hua, ZHANG Zhou, XU Bin*Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 200031, P.R.China;Alpha-Anordrin Induced Differentiation of Swarm Rat Chondrosarcoma[J];Chinese Journal of Cancer;2001-08
5 LIN Wei 1* ,SONG Jin-dan 1 ,LIN Jian-yin 2 1.Department of Cell Biology,China Medical University,Shenyang 1100 01,P.R.China2.Department of Molecular Medicine,Fujian Medical University ,Fuzhou35004,P.R;Nitric Oxide Affects Proliferation of Stomach Cancer Cell Line BGC- 823[J];Chinese Journal of Cancer;2001-10
6 WANG Yi-Ping1, ZHANG Cai1,2, NIU Jia-Feng1, ZHANG Jian-Hua1, XU Xiao-Qun1, WANG Jun-Fu11. Institute of Basic Medicine, Shandong Academy of Medical Sciences, Jinan, Shandong, 250062, P. R. China2. Institute of Immunopharmacology and Immunotherapy, School of Pharmaceutical Sciences,Shandong University, Jinan, Shandong, 250012, P. R. China;Expression and Significance of NKG2D Ligandsin 13 Tumor Cell Lines[J];Chinese Journal of Cancer;2008-03
7 XU Zhuo-li GUO Jun-hua WANG De-wen WU De-zheng (Centre of Cancer Research, North Taiping Road Hospital Beijing · 100039 );THE RELATIONSHIP BETEWEEN THE LEVEL OF METALLOTHIONEIN AND PATHOLOGICAL DAMEGE INDUCED BY CISPLATIN IN THE KIDNEY OF MICE[J];CHINESE JOURNAL OF CANCER;1995-06
8 Chen Zhiyun,Mo Yunsong,Xiang Baikang,et al Hospital Affiliated to Zhejiang College of TCM,Hangzhou(310006);Weierkang Granule Treat Chronic Atrophic Gastritis[J];Journal of Zhejiang College of Tcm;2003-03
9 Hu Yin,Yin Weigang,Xu Zuoliang  (Department of Basic Medicine,Baotou Medical College,Baotou 014010,Beijing Medical University );MTT Colorimetric Assay for Testing Cytotoxic Activity of MGC 803 T[J];JOURNAL OF BAOTOU MEDICAL COLLEGE;1998-03
10 WANG Yi-rui~(1,2),XIAO Jun-jun~2,DONG Xiao-min~2,MENG Shu-cong~2,DENG Sheng-ju~1,KUANG Bin~1,YAN Jun~1,ZHAO Fang~1,ZENG Hui-hui~(1△)(1.Deparptment of Chemical Biology,School of Pharmaceutical Sciences,2.Department of Cell Biology,School of Basic Medical Sciences,Peking University,Beijing 100083,China);Immune regulating activity of a novel organoselenium compound ethaselen-1 in C57/BL mice[J];Journal of Peking University(Health Sciences);2006-06
China Proceedings of conference Full-text Database 2 Hits
1 DAI XUAN-YING~1 XU SHI-QING~(1*) CHEN XI-LIN~1 YI HONG-GEN~2 LIU XUE-GUANG~1 WANG YU-JUN~1 ~1School of Agricultural Science and Technology & Institute of Sericulture,Soochow University.Suzhou 215006,P.R.China ~2Department of science research,Soochow University,Suzhou 215006,P.R.China;Effect of Environmental Hormone Atrazine on Insect Culture Cells[A];[C];2004
2 Jiang Bei, Sun Han-Dong Laboratory of Phytochemistry, Kunming Institute of Botany, The Chinese Academy of Sciences, Kunming 650204;Studies on the Chemical Constituents and Bio-activities of Isodon sculponeata[A];[C];2001
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