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《Chinese Journal of Cancer》 1992-04
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CAI Hai-ying YE Yan- li LU Sheng-xian (Cancer Institute, Sun Yat-sen University of Medical Sciences.Guangzhou. 5 10060)  
The DNA content of the epithelium cells and the characteristics of cell proliferation cycle of various phase of malignant transformation were analysed by flow-cytometry. Comparison has been made amony three cell lines withdifferent proliferation capacityf33the normal esophgeal epithelium(NRE),the anaplasticepithelium induced by NSEE(DRE) and RE25 -3 (malignant transformation cell from DRE) Without using carcinogen in vitro .The experimental results showed that, NRE cells and DRE :ells have similar DNA content, DI-1.0, while the DI of various generations of the kE25-3 cells incerased to 1.60--3.36. The analysis of the cell proliferation cycle showed that the percentage of S phase cells was 5.04% in NRE, 29.92% in DRE, while in R-E5-3 it increased froml0.62%to 45.85% much higher than NRE and DRE. When RE25-3 was transplanted to the nude mice (RE25/N) its S phase cell decreased markedly which impress that the proliferation capacity of the cell may be affected by the immunity of the host.
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