IN VITRO RADIOSENSITIZATION OF CHINESE HERB SALVIA MILTIORRHEA MIXTURE, PHERETIMA ASIATICA, AND STAUNTONIA CHINENSIS ON CULTURED HUMAN NASOPHARYNGEAL CARCINOMA CELL LINE
CAO Qi-yuan LI Yong-qiang (Cancer Institute, Sun Yat-sen University of Medicd Sciences, Guangzhou. 510060 )CHEN Cheng-qin WU Yu-zheng LIANG Jian-xiong (Tumor Hospital, Sun Yat-sen University of Medical Sciences, Guangzhou, 510060 )
The radiosensitization activity of Chinese herbal medicines Injectio Sal- via mitltiorrhiza complex, Pheretima asiatica, and Stauntonia chinensis on cultured human na-sophary ngeal carcinooma cell line (CNE-2) in vitro was studied. The CNE-2 cells were seeded into 96 well microcultured plate, 50 cells per well, 24 wells as one group with or without medicine in media, cultured for 12-15 hrs. Then the cells were irradiated by 60Co in a obse of 2 Gy y-ray and continuously cultured for 70-72 hrs. The cell clones(≥8 cells) per well were observed and counted by inverted microscope. The inhibition rate of colony formation in the irradiated combined with medicine group increased by 27.05%-54. 27% over the irradiated alone group. The differences were statistically highly significant(P0.0001). Using methylene blue colorimetric method to assay the O2 consumption, the results showed that all of the three Chinese herbal medicines can promote apparently O2 consumption of CNE-2 cells.