Full-Text Search：
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《CHINESE JOURNAL OF CANCER》 1999-05
Add to Favorite Get Latest Update

Studies on arsenic trioxide induced human pulmonary adenocarcinoma GLC-82 cell apoptosis and its molecular mechanisms

DENG You ping, LIN Chen, ZHANG Xue yan, et al.National Laboratory of Molecular Oncology,Cancer Institute,Chinese Academy of Medical Sciences,Peking Union Medical College, Beijing 100021, P.R. China  
Objective:To study the biological effect of As2O3 on human pulmonary adenocarcinoma GLC 82 cells and its mechanisms.Methods:MTT reduction assay,morphology investigation, flowcytometric analysis, DNA gel electrophoresis and TUNEL,RT PCR, Northern blot, Western blot methods were perfomed. Results:As2O3 inhibited the growth of GLC 82 cell line. The cells treated with As2O3 showed a typical apoptotic morphology and hypodiploid peak before G1 phase.DNA of treated GLC 82 cells appeared a ladder pattern characteristic of apoptosis.In situ cell death detection analysis also revealed the DNA fragmentation. Besides, As2O3 inhibited c myc gene expression and increased p53 and p16 gene expression. Conclusion:As2O3 can induce GLC 82 cell apoptosis mainly with regulation of c myc,p53 and p16 gene expression.
【Key Words】： Arsenic trioxide Human pulmonary adenocarcinoma GLC 82 cells apoptosis Gene expression
【CateGory Index】： R730.231
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【References】
Chinese Journal Full-text Database 10 Hits
1 YAO Hua 1, WANG Guo quan 1, ZHU Bin 2, SUN Yue 2, JING Feng xiang 2, ZHAO Jian long 2 (1.Teaching and Research Section of Environmental Hygiene, Xinjiang Medical University, Urumqi, Xinjiang 830054, China; 2.Shanghai Institute of Metallurgy, Chinese Academy of Sciences, Shanghai 200050, China);The Study on Several Tumor Genes Expression Caused by NaAsO_2 in Liver Cells[J];Endemic Diseases Bulletin (China);2002-04
2 NIE Lin, ZHANG Yuan(Institute of Hematology, MCJUMC, Guangzhou 510632, China);General feature of arsenic therapeutic mechanism(A review)[J];Journal of Jinan University(Natural Science & Medicine Edition);2001-04
3 FAN Xiang shan,ZHANG Dong sheng (Department of Pathology and Pathophysiology,School of Basic Medical Science,Southeast University,Nanjing 210009,China);A summary on apoptosis of malignant tumor cells induced by arsenic trioxide[J];Journal of Southeast University;2003-03
4 HUANG Shou guo,JIANG Sen.Department of Obstetrics and Gynecology,Qilu Hospital of Shandong University,Jinan 250012,China;Experimental Study on Chemotherapy of Neoplasms by Arsenic Trioxide[J];Journal of Qilu Oncology;2002-03
5 ZHANG Peng,LIU Hai-jiang,LIU Yan-ping.the First Medical College of Harbin Medical University,Harbin 150001,China;Studies on Effect of Arsenical on Tumors[J];Journal of Qilu Oncology;2003-11
6 YAO Hua, WANG Guo quan, ZHU Bin, et al (Department of Environmental Hygiene, Xinjiang Medical University);Effect of sodium arsenite to the level of twenty genes expression[J];Journal of Xinjiang Medical University;2001-01
7 WANG Yu-ling, QI Jun-sheng (Chongqing Three Gorges University, Chongqing 404000, P.R.C.);A summary on anti-tumor molecular mechanisms of arsenide[J];Journal of Southwest University for Nationalities (Natural Science Edition);2005-04
8 LI Zhi-Hui1,2, LI Hui-Min1,LI Bin1,LI Xiao-Jin11Department of Hematology,Yunnan Provincial Second People Hospital,Kunmin 650021,Yunnan Province,China; 2Beijing Daopei Hospital,Beijing 100049,China;Effect of Arsenic Trioxide on Apoptosis in Lymphoblastoid Raji Cell Line with Relation to Expression of Mcl-1 Gene[J];Journal of Experimental Hematology;2009-03
9 NIE Lin, ZHANG Yuan,ZHANG Jian jun, et al (Department of Oncology, First Affilliated Hospital ,Medical College of Jinan University, Guangzhou Guangdong 510630, China);Combinational effects of bcl-2 antisense oligodexynucleotide with arsenic trioxide on malignant lymphoma cell lines[J];China Oncology;2002-03
10 ZHU Zhaohui *,LIN Chen,ZENG Fuqing,et al. *Department of Urology, Union Hospital, Tongji Medical College,Huazhong University of Science and Technology, Wuhan 430022,China;An experimental study of combining Ad-p16 with chemotherapy agents on human bladder cancer cells[J];Chinese Journal of Urology;2002-10
【Citations】
Chinese Journal Full-text Database 2 Hits
1 ZhangPeng,WangShuye,HuLonghu,etal.FirstClinicalMedicalColegeofHarbinMedicalUniversity,Harbin,150001;Treatmentofacutepromyelocyticleukemiawithintravenousarsenictrioxide[J];CHINESE JOURNAL OF HEMATOLOGY;1996-02
2 Lu Yongjie; Cheng Shujun ; Guo Suping ;et al. (Departmeat of Etiology, Cancer institute,ChineseAcademy of Medical Sciences, and Peking Union Medi-cal College, Beijing 100021);Detection of c-inyc translocation in human lung cancercells by FISH[J];NATIONAL MEDICAL JOURNAL OF CHINA;1995-04
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 ZHANG Chen, HUANG Shi lin, XIANG Yang(The Institute of Traditional Chinese Medicine Hematology,the??210th Hospital, PLA, Liaoning Dalian 116021, China);Effect of Realgar on Apoptosis in Raji Cells[J];Journal of Anhui Traditional Chinese Medical College;2003-02
2 LI Jiang tao1＊ , OU Qing jia1, WEI Jing2 1.Department of Liver Surgery,Sun Yat sen Memorial Hospital, Sun Yat sen University of Medical Sciences,Guangzhou 510120, P.R.China 2.Research Center of Medicine, Sun Yat sen Memorial Hospital, Sun;Studies on arsenic trioxide induces apoptosis in hepatoma cell lines Bel-7402[J];Chinese Journal of Cancer;2000-12
3 HE Zhi jie1*, ZHU Xing min2, CAI Wei bo1, CHEN Zhao hua3, XU Hong xing3, WU Yuan jian31.Department of Hematology, Suzhou Second Peoples Hospital, Suzhou 215002, P.R.China2. Department of Hematology, Affiliated Children Hospital of Suzhou Universit;Experimental Study on Apoptosis and Necrosis of HumanLeukemia Cell Line HL-60 Induced by As_2S_3[J];Chinese Journal of Cancer;2001-08
4 JIA Pei min, ZHU Qi, YU Yun, CHEN Guo qiang, CHEN Sai juan, CHEN Zhu, WANG Zhen yi, TONG Jian hua*.Shanghai Institute of Hematology,Ruijin Hospital,Shanghai Second Medical University,Shanghai 200025,P.R.China;Experimental Study of Low Dose Arsenic Trioxide in Treatment of Patients with Acute Promyelocytic Leukemia[J];Chinese Journal of Cancer;2002-04
5 HUANG Shou guo*, KONG Bei hua, YANG Rui fang, JIANG Sen Department of Obstetrics and Gynecology,Qilu Hospital of ShandongUniversity,Jinan 250012,P.R.China;Primary Study of Arsenic Trioxide Inhibits Abdomino-MetastaticTumor Formation of Human Ovarian Carcinomain Nude Mice and Its Mechanisms[J];Chinese Journal of Cancer;2002-04
6 ZHANG Yao,ZHAO Yan,CHEN Chang jie (Department of Biochemistry and Molecular Biology,Bengbu Medical College,Anhui 233003 ,China);Inhibitory effects of arsenic trioxide on telomerase activity in HL-60 cells induced[J];Journal of Bengbu Medicinae College;2002-01
7 ZHANG Yao,ZHAO Yan,CHEN Chang jie(Department of Biochemistry and Molecular Biology,Bengbu Medical College,Anhui 233003,China);The effect of arsenic trioxide on the growth of HL-60 cell[J];Journal of Bengbu Medicine College;2003-02
8 ZHANG Man ying,AN Ji hong,LIU Xiao qiu (Dept.of Respiration,the Seconed Hospital,Jilin University,Changchun 130041 China);Apoptosis of Spc-A_1 cells induced by As_2O_3 and the effect on cell cycle[J];Journal of Norman Bethune University of Medical Science;2001-06
9 Cheng Jin; Wang Yaoping; Xue Huiliang; et al(Xinhua capital Affliated to Second Shanghai Medical University(Shanghai, 200092));TREATMENT OF REFRACTORY ACUTE PROMYELOCYTIC LEUKEMIA WITH ARSENIC TROXIDE[J];Journal of Leukemia;1998-03
10 Wang Shuye,Xiao Yujun,Geng Jie,et al First medical college of Harbin medical university.(Harbin,150001);THE ELEMENTARY INVESTIGATION ON DRUG RESISTANCE OF ARSENIC TRIOXIDE(As_2O_3) IN TREATMENT OF ACUTE PROMYELOTIC LENKEMIA(APL)[J];Journal of Leukemia;1999-06
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 ZHANG Ai-hua, YANG Guang-hong, LI Jian, WANG Rong Department of Prevetive Medicine, Guiyang Medical College, Guiyang 550004, China;THE SITUATION OF DNA SYNTHESIS、 DNA DAMAGE AND DNA REPAIR IN ARSENISM PATIENTS BLOOD CELLS CAUSED BY COAL-BURNING[J];CARCINOGENESIS, TERATOGENESIS AND MUTAGENESIS;2000-02
2 WANG Wei 1,2 ,WU Yong-jun 1 ,WU Yi-ming 1,ˇ (1.Department of Occupational Health and Health Toxicology,College of Public Health,Zhengzhou University,Henan Zhengzhou450052, China;2.Zhengzhou Institute of Occupational Disease, Zhengzhou Henan450053,China);Genetic Polymorphism in hOGG1 and Susceptibility to Lung Cancer[J];Carcinogenesis,Teratogenesis and Mutagenesis;2005-02
3 ZHANG Chen, HUANG Shi lin, XIANG Yang(The Institute of Traditional Chinese Medicine Hematology,the??210th Hospital, PLA, Liaoning Dalian 116021, China);Effect of Realgar on Apoptosis in Raji Cells[J];Journal of Anhui Traditional Chinese Medical College;2003-02
4 Sun Yueping;Chen Guoqiang;Cai Xun,et al(Shanghai Institute of Hematology, Rui-Jin hospital.Department of Biology,Shanghai Second Medical University (Shanghai 200025);Phenylarsine oxide induces apoptosis in acute promyelocytic leukemia cell line NB_4[J];CHINESE JOURNAL OF CANCER;1999-01
5 ZHAO Wei hong, CHEN Jia pei, CONG Yu wen,et alInstitute of Radiation Medicine, Beijing 100850,P.R.China;Caffeine promoting tumor cells apoptosis induced by irradiation[J];CHINESE JOURNAL OF CANCER;1999-04
6 SUN Yue-ping, JIA Pei-min, CAI Xun, et al. ( Rui-jin Hospital, Shanghai Second Medical University, Shanghai 200025, P. R. China Department of Biology, Shanghai Second Medical University, Shanghai 200025, P. R China);In vitro effects of phenylarsine oxide on hematopoietic malignant cell lines[J];CHINESE JOURNAL OF CANCER;2000-01
7 ZHANG Jian-jun, FAN Zhen-bin, CHENG Shu-jun, et al. (Cancer Institute (Hospital), Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, P. R. China);Potential of microsatellite analysis in detection of bladder cancer in the Chinese[J];CHINESE JOURNAL OF CANCER;2000-06
8 HE Dong-mei ; ZHANG Huan (Institute of Hematology, Jinan University Medical College , Guangzhou 510632, P R China);Effect of hTERT antisense oligodeoxynucleotide on telomerase activity in K562 cells[J];CHINESE JOURNAL OF CANCER;2000-07
9 LI Jiang1＊ ， YU Xin xin2， ZHENG Jian yong1， et al. 1.Department of Pathology, Fourth Military Medical University, Xi'an 710033, P.R.China 2.State Key Laboratory for Molecular Virology and Genetic Engineering, Beijing 100052, P.R.China;Overexpression of Bak gene may lead to apoptosis in hepatocellular carcinoma[J];CHINESE JOURNAL OF CANCER;2000-09
10 RONG Yu＊ , LIANG Fu you, CHEN Li, DU Hai jun, LIU Le yao, SUN Hong liu, AN Wei Department of Cell Biology, Capital University of Medical Sciences, Beijing 100054, P.R.China;Norcantharidin induces apoptosis in human breast cancer cell line[J];Chinese Journal of Cancer;2000-12
【Secondary References】
Chinese Journal Full-text Database 10 Hits
1 WANG Qi-lin et al (School of Life Science,Liaocheng University,Liaocheng,Shandong 252059);Study on the Apoptosis Mechanism of Tumor Cells Induced by Laminarin[J];Journal of Anhui Agricultural Sciences;2009-09
2 MA Jia,CHEN Su-lian,CHEN Zhi-wen (Department of Biochemistry and Molecular Biology,Bengbu Medical College,Anhui 233003,China);Effect of arsenic trioxide on induction of apoptosis of melanoma A_(375) cells[J];Journal of Bengbu Medical College;2004-06
3 ZHANG Chen, LING Chang-Quan* (Department of Traditional Chinese Medicine, Changhai Hospital, Second Military Medical University .Shanghai 200433 ,China);Review of arsenic trioxide effect on solid cancer[J];Academic Journal of Second Military Medical University;2003-04
4 LIANG Jun , ZHAN Shu-Jun, ZHAO Yuan-Yuan, SHEN Fang-Zhen, YAO Ru-Yong,DING Ai-Ping, SHANG Qing-Jun (Department of Oncology, The Affiliated Hospital, Medical College,Qingdao University, Qingdao 266003, China);Anti-tumor effect and mechanisms of arsenic trioxide on subcutaneously implanted gastric cancer in 615 in-breeding mice[J];Academic Journal of Second Military Medical University;2004-08
5 YANG Shi ming,FANG Dian chun,LUO Yuan hui, LU Rong, LIU Wei wen (Department of Gastroenterology, Southwest Hospital, Third Military Medical University,Chongqing 400038,China);Effects of arsenic trioxide on telomerase activity and cell cycle of gastric cancer cell line SGC-7901[J];Acta Academiae Medicine Militaris Tertiae;2001-09
6 GAO Shuang,GONG Hui-zhi,JIANG Hong,LI Bing,LI Xin.China Medical University,Shenyang 110001,China;Effects of Arsenic on oxidative damage in primary cultured rat hippocampal neurons[J];Chinese Journal of Control of Endemic Diseases;2011-01
7 SU Ying, SHEN Shiren, GAO Pin, ZOU Changyan, CHEN Zeng. Radiobiology Department, Fujian Tumor Hospital, Fuzhou, Fujian 350014, P.R.China;A study on apoptosis induced by As_2O_3 for small cell lung cancer[J];Chinese Journal of Lung Cancer;2001-06
8 WANG Zhaoxia, LU Binbin, WANG Teng, DE Wei, SHU Yongqian. Cancer Center, The First Affiliated Hospital, Nanjing Medical University, Nanjing, Jiangsu 210029, P.R.China;Effect of recombinant adenovirus-p53 on growth and chemosensitivity of human lung adenocarcinoma cell lines[J];Chinese Journal of Lung Cancer;2006-02
9 GUO Li-xia,WANG Yuan-liang,XIN Juan nistry of Education, College of Bioengineering, Chongqing University, Chongqing 400030, China);Reserarch advance on mechanism of arsenic trioxide on tumor[J];Journal of Chongqing University(Natural Science Edition);2004-08
10 HAO Chun-li1,ZHAO Li1 and ZHUANG Zhi-xia1,2(1.Testing Lab of Xiamen Huaxia Vocational College,Xiamen 361024;2.Department of Chemistry,Xiamen University,Xiame 361005);Analysis of arsenic speciation in traditional Chinese medicines by hyphenated technique of high performance liquid chromatography-inductively coupled plasma mass spectrometry[J];Chinese Journal of Analysis Laboratory;2011-06
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved