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Immunogenicity and biological characteristics of CD80 transfected B16 melanoma

LIU Ran yiΔ, XIONG Yu fang,QU Shen, et al.Department of Biochemistry,Tongji Medical University,Wuhan 430030,P.R.ChinaΔBiotherapy Laboratory,Cancer Center,Sun Yat sen University of Mendical Sciences, Guangzhou 510060,P.R. China  
Objectives:To investigate the effects of the CD80 costimulatory molecule expression on the immunogenicity and biological characteristics of poorly immunogenic B16 melanoma cells, explore the relationship between the immunogenicity of tumor cells and cell surface molecules. Methods:Human CD80 cDNA was transfected into B16 melanoma cells by lipofectin mediated gene transfer before the expansion of tumor cells were tested by MTT method in vitro;C57BL/6 mice were inculated subcutaneously either mock transfected B16 cells (B16 neo)or CD80 transfected B16 cells (B16 CD80),then the latency,survival times and tumor mass growth were investigated.The lymphocytes were examined for both proliferation indices (PI) and specific cytotoxic activity by MTT method and improved LDH releasing method,after syngenic Mixed Lymphocyte tumor cultures (MLTCs). Results:In the B16 CD80 cells,in which CD80 expression were detected by SABC method, demonstrated slower growth rate than B16 neo Clles in vivo (P 0 05) , though they didn't in vitro.Compared with B16 wt and B16 neo cells, B16 CD80 cells more effectively induced the proliferation of effector lymphocytes and the generation of specific lytic activity against B16 wt cells. Conclusions:The CD80 (B7 1) expression in poorly immunogenic tumor cells can increase their immunogenicity and decrease their tumorigenicity,the effects are associated with the expression of ICAM 1 and MHC molecules on tumor cells surface.
【Fund】: 国家教委基金!资助课题(NO.JW9307)
【CateGory Index】: R730.3
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