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《CHINESE JOURNAL OF CANCER》 1999-06
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The effect of RA and DEX on the differentiation and telomerase activity of cultured osteosarcoma cells

WANG Lin, WEN Jian ming, ZHANG Meng, et al. Department of Pathology, Sun Yat sen University of Medical Sciences, Guangzhou 510080,P.R.China  
Objective:To investigate the relationship between morphological and functional induction of differentiation and the expression levels of telomerase activity in osteosarcoma cells. Methods:Osteosarcoma cells were treated with all trans retinoic acid (RA) and dexamethasone (DEX) for the induction of differentiation. The morphological and functional changes of the cells were observed. Cell cycle of the cells was determined by flow cytometry. Telomerase activity of treated cells was detected by TRAP method. Results:RA and DEX can arrest the cell cycle of cultured HOS cells at G 2/M phase. After treatment with RA or DEX, the growth of HOS cells was inhibited. The cells underwent morphological and functional differentiation. Alkaline phosphatase activity of the cells increased significantly more than before. The differentiated HOS cells showed a dose and time dependent decline of telomerase activity. Conclusions:Both RA and DEX could inhibit cell growth and induce differentiation of HOS cells morphologically and functionally. The decrease of telomerase activity can be considered as an indicator of differentiation. These results suggest, therefore, that telomerase regulation directly links to cell differentiation rather than cell cycle.
【Fund】: 中华医学基金会 (CMB)综合发展项目资助 !( 1998年No .3 )
【CateGory Index】: R730.231
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