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《CHINESE JOURNAL OF CANCER》 1999-06
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Detection of the hypermethylation of calcitonin gene by polymerase chain reaction and its implication in some hematological malignancies

WANG Shun qing *,HONG Wen de,PENG Ai hua,et al. *Department of Hematology,Guangzhuo First Municipal Peop les Hospital, Guangzhou 510180,P.R.China  
Objective:To study the methylation pattern of calcitonin gene (CT gene) in acute nonlymphoblastic leukemia (ANLL ),myelodysplastic syndrome (MDS) and chronic myeloid leukemia (CML) and its implication. Methods:Restriction endonuclease and polymerase chain reaction (PCR) techniques were used. Results:The hypermethylation pattern was found in 72%(18/25) of ANLL and 60%(6/10) of MDS patients.In CML,23.8%(5/21) samples in chronic phase and 80%(8/10) samples in blast crisis were positive ( P 0. 05) . And the sensitivity of this method was at least 3 logs (1 in 1000). Conclusion:This findings indicated that the hypermethylation of CT gene was a common molecular marker for the tumor cells in ANLL,MDS and the progression and blast crisis of CML.The hypermethylation of CT gene might be associated with the transformation to increase blastic cells in MDS and might have prognostic value in MDS.
【CateGory Index】: R730.263
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