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Detection of the hypermethylation of calcitonin gene by polymerase chain reaction and its implication in some hematological malignancies

WANG Shun qing *,HONG Wen de,PENG Ai hua,et al. *Department of Hematology,Guangzhuo First Municipal Peop les Hospital, Guangzhou 510180,P.R.China  
Objective:To study the methylation pattern of calcitonin gene (CT gene) in acute nonlymphoblastic leukemia (ANLL ),myelodysplastic syndrome (MDS) and chronic myeloid leukemia (CML) and its implication. Methods:Restriction endonuclease and polymerase chain reaction (PCR) techniques were used. Results:The hypermethylation pattern was found in 72%(18/25) of ANLL and 60%(6/10) of MDS patients.In CML,23.8%(5/21) samples in chronic phase and 80%(8/10) samples in blast crisis were positive ( P 0. 05) . And the sensitivity of this method was at least 3 logs (1 in 1000). Conclusion:This findings indicated that the hypermethylation of CT gene was a common molecular marker for the tumor cells in ANLL,MDS and the progression and blast crisis of CML.The hypermethylation of CT gene might be associated with the transformation to increase blastic cells in MDS and might have prognostic value in MDS.
【CateGory Index】: R730.263
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Chinese Journal Full-text Database 3 Hits
1 ;Epigenetic regulation for prevention and gene therapy of tumors[J];Journal of Xinxiang Medical College;2004-06
2 TANG Yiping, DENG Chengqi, DU Qiong, et al. (Sichuan Provincial People's Hospital, Chengdu 610072, China);Hypermethylation of the calcitonin gene as molecular genetic marker in detecting minimal residual disease of leukemia[J];Chinese Journal of Internal Medicine;2001-04
3 WU Hongxing 1, LAI Wenying 1, DENG Xianghong 1, LIU Dihui 1, LI Ming 1, RUAN Jing 1, HUANG Liangjin 1, LUO Xuequn 2(1 Department of Pediatrics, Zhongshan Hospital Affiliated to Sun Yat-sen University School of Medicine, Zhongshan 528403, China; 2 Department of Pediatrics, the First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, China);MS-PCR Monitoring the Minimal Residual Disease of Children Acute Non-Lymphocytic Leukemia in Bone Marrow and Peripheral Blood[J];Clinical Medicine & Engineering;2012-09
Chinese Journal Full-text Database 10 Hits
1 Yuan Zhenhua, Ding Youchang, Chen Xiaojun, Wang Mingfa, Yang Zhangping Hangzhou Sanitation and Anti epidemic Station, Hangzhou\ 310006;STUDY ON THE SAFETY OF INDOOR ORNAMENTAL MATERIALS[J];CARCINOGENESIS, TERATOGENESIS AND MUTAGENESIS;1999-05
2 BAI Zhi Yong 1, XU Guo Bin 2, WU Shu Lan 1 (1. Department of Hematology, 2. Department of Laboratory, the First Hospital, Beijing Medical University, Beijing 100034, China);Detection of DNA-methyltransferase activity of leukemia cells with radiology microassay[J];Journal of Beijing Medical University;2000-01
3 GONG Yan-bing,ZHONG Ming,WANG Jie.(Department of Pathology,College of Stomatology,China Medical University,Shenyang 110002,P.R.China);Epigenetic Code is Chaos in Cancer Cell[J];International Journal of Genetics;2006-02
4 LIU Jun_zhuo,HAO Lan_ying,WEN Tian_you, et al. Department of Occupational and Environmental Health, School of Public Health,Peking University,Beijing 100083.;Indoor Decoration and Sick Building Syndrome[J];Journal of Environment and Health;2002-01
5 LU Rong,FANG Jing-Yuan*(Shanghai Institute of Digestive Disease,Shanghai Jiaotong University Medical School,Renji Hospital,Shanghai 200001,China);Epigenetic modification and cancer[J];Chinese Bulletin of Life Sciences;2006-01
6 He,Zhong-Xiao;Gu, Yu;Rao, Hong;Bai,Jian-Shi; Ma, Qing (Department of Biology, Beijing Normal University, Beijing 100875);The Effect of 5-Aza-2'-deoxycytidine (5-Aza-CdR)on Cell Differentiation and DNA Methylase of HL-60 Cells[J];;1995-04
7 ZHANG Yong-Biao, CHU Jia-You(Department of Genetics, Institute of Medical Biology, Peking Union Medical College, Kunming 650118, China);Progress of Research on Epigenetic and Human Disease[J];Hereditas(Beijing);2005-03
8 ZHANG Yongping, ZHANG Xiaochun, ZHENG Chuanjing, et al Department of Pediatrics, Affiliated Hospital, Ningxia Medical College,Yinchuan 750004;Logistic regression analysis of risk factors in child leukemia[J];Chinexe Journal of Pediatrics;1999-10
9 Qiu Dekai; Shen Lanlan; Fang Jingyuan; et al.(Shanghai Institute of Digestive Diseases, 200001);Change of total genomic DNA methylation level in human hepatocellular carcinoma[J];CHINESE JOURNAL OF DIGESTION;1997-05
10 YE Yukun(叶玉坤),SU Changqing(苏长青),WANG Dong(汪栋),GUO Weixing,CHENG Xing,LIU Shuqiu(刘菽秋),LIU Yanjun(刘彦君),LIU Bolin(刘柏林),CAO Xiangrong(曹祥荣),SHAN Xiangnian(单祥年)and WU Mengchao(吴孟超);Relations of tumor suppressor gene p16 and RB to early diagnosis of lung cancer[J];Chiese Journal of Surgery;2000-12
【Secondary References】
Chinese Journal Full-text Database 3 Hits
1 YUAN Yun fei 1, LI Jin qing1, YANG Zu li2, WANG Jian ping2*1. Tumor Hospital, Sun Yat sen University, Guangzhou 510060, P.R.China.2. The First Affiliated Hospital, Sun Yat sen University 510089, P.R.China;The Roles of DNA Methylation in Human Neoplasms[J];Chinese Journal of Cancer;2002-11
2 XU Shuo-qi(Experimental Center of Life Science,Nanjing Agricultural University,Nanjing 210095,China);Epigenetics in Tumor[J];Animal Husbandry and Feed Science;2010-02
3 ZHU Hang,LEI Xun,ZHANG Fan,WANG Yang Maternal,Child and Adolescent Health Care Teaching Section,Public Health and Administration School,Chongqing Medical University,Chongqing 400016,China;Risk Factor and Population Attributable Risk of Children Leukemia in China:A Meta-Analysis[J];Chinese Journal of Evidence-Based Medicine;2012-10
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