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The clinical significance of deletion of p16 gene homozygous deletion in malignant pleural effusion

GUI Shuyu 1、2 , WANG Yuan1, LIU Hu1, et al 1 Laboratory of Molecular Biology and Department of Biochemistry, Anhui Medical University, Hefei 230032, PRChina 2 Department of Respiratory Medicine, the First Affiliated Hospital of Anhui Medical Uni  
Objective:To investigate the clinical significance ofhomozygous deletion of p16 gene in malignant pleural effusionMethods:The homozygous deletion of p16 gene exon1 and exon2 was examined by polymerase chain reaction (PCR) in 31 specimens of pleural effusions from lung cancer patients and compared with the determination of exfoliated cell in same specimens fromwhich the clinical value was analysed in clinical diagnosisResults:No homozygous deletion of p16 gene exon1 was found in 31 malignant pleural effusion samplesThe homozygous deletion of p16 gene exon2 was detected in 12 sampls from31 pleural effusions, while cytology positivity was found in 16 of all examined pleural effusionsThe positive rate in 31 malignant pleural effusion samplesby the determination of homozygous deletion of p16 gene and the examination of exfoliated cell was 3871%(12/31) and 5162%(16/31), respectivelyThe positive rate could be enhanced from 5162(16/31) to 7097%(22/31) by using both ofabove methods, There was no significnt difference of positive rate between homozygous deletion of p16 gene exon2 and exfoliated cytology The overall positive rate of both examination was higher than that of only the determination of homozygous deletion of p16 gene exon2 or exfoliated cytology in malignant pleural effusionNo homozygous deletion of p16 gene was found in 21 tuberculosis pleural effusion samplesConclusions:Our present data suggest that combining examinations of exfoliated cytology and homozygous deletion of p16 gene exon2 in pleural effusion can not only increase the positive rate of diagnosis, but also recruit and enhance the diagnostic value of conventional pleural cytology
【Fund】: 安徽省教委自然科学研究基金!(96-JL077);; 安徽医科大学附属医院科研基金资助
【CateGory Index】: R730.4
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Chinese Journal Full-text Database 1 Hits
1 LI Qian-ping,YANG Hong-sheng(Dept.of Thoracic Surgery,The 1st Affiliated Hospital of Kunming Medical College,Kunming 650032,China);Lung Cancer Related Gene p16 and CyclinD1[J];Academic Journal of Kunming Medical College;2005-03
Chinese Journal Full-text Database 8 Hits
2 Yang Jun \ Liuzhou Worker's Hospital, Guangxi(545005) \ \;Study on carcinoembryonic antigen and ferritin in benign and malignant pleural effusion[J];GUANGXI MEDICAL JOURNAL;1998-03
3 SHEN Wen-fu,LIAO Gen-yun,YIN Kai-sheng (The People's Hospital of Jianhu,Jiangsu Province 224700,China);Study on diagnostic value of analyzing CYFRA21-1 combined with CEA in benign and malignant pleural effusions[J];Hainan Medical Journal;2004-04
4 WANG Li-hua ,LU Zhong-qin ,DOU Hai-yan (The and Hospital of Chifeng City,Neimeng Chifeng 024000, China)$$$$;The value of using CEA and LDH of BALF to recognize and diagnose benign and malignant pulmonary disease[J];Hebei Medicine;2002-02
5 Sun Xiao;Wang Lin;Guo Changzheng;et al(266003 QingDao NO.2People's Hospital department of internal medicine);A STUDY ON CEA DETERMINATION IN BALF SERUM AND URINE TO DIAGNOSIS FOR LUNG CANCER[J];HEBEI MEDICINE;1995-05
6 Li xiaohui Zhuo Gangyi.et al. Respiratory department,The Shichuan provincial people's hospital,Chengdu 610072;The value of pleural adenosine deaminase and ratio of pleurallysozyme to serum lysozyme in the diagnosis of tuberculous pleural effusions[J];Journal of Clinical Pulmonary Medicine;1997-04
7 YANG Jing ping, SUN De jun, WANG Xiu xiang, et al. (Department of Respiratory Disease, Third Affiliated Hospital, Inner Mongolia Medical College, Baotou 014010 China);THE IMPLICATION OF MULTIPLE ASSAYS IN DIFFERENTI-ATION OF BENIGN FROM MALIGNANT HYDROTHORAX[J];Acta Academiae Medicinae Neimongol;2003-02
8 DOU Hai yan,WANG Li hua,Gerile,LING Zhi (Department of Respiratory,The 2nd hospital of Chifeng city,Chifeng 024000,China) [;The value of using CEA and LDH to recognize and diagnose benign and malignant pleural effusion[J];Nei Moivgol Medical Journal;2000-05
Chinese Journal Full-text Database 10 Hits
1 LIU Jin yi, AN Qian, SUN Wen yue, HUANG Jin feng, PAN Qin jing, CAO Jia, CHENG Shu jun, GAO Yan ning (Department of Hygiene Toxicology, College of Military Preventive Medicine, Third Military Medical University, Chongqing 400038, China);Analysis of p16 hypermethylation in sputum samples from lung cancer patients[J];Acta Academiae Medicinae Militaris Tertiae;2003-19
2 JIN Yongtang , ZHOU Xiaotie, HE Xingzhou. Department of Environmental Health, Anhui Medical University, Hefei, Anhui 230032, P.R.China;The general measurement of genetic factors on lung cancer in Xuanwei, China[J];Chinese Journal of Lung Cancer;2001-05
3 JIN Yongtang , HE Xingzhou, ZHOU Xiaotie. Department of Environmental Health, Anhui Medical University, Hefei, Anhui 230032, P.R.China;Analysis of the familial risk factors on lung cancer[J];Chinese Journal of Lung Cancer;2002-02
4 WANG Xiaomei, SHAN Jun, CHEN Zhuohuai. Department of Pathology, Department of Radiology, Shenzhen People's Hospital, Shenzhen, Guangdong 518020, P.R.China;An experimental study on the association of cyclin D_1 oncoprotein with mutated p53 in progressive development of pulmonary tumors[J];Chinese Journal of Lung Cancer;2002-02
5 JIAO Xianfa *, WANG Guobin, QIN Jianling, WANG Feng, WANG Ning. * Zhengzhou Fifth People Hospital, Zhengzhou, Henan 450003, P.R.China;The clinical significance of detection of telomerase in bronchoalveolar lavage liquid in patients with lung cancer[J];Chinese Journal of Lung Cancer;2002-04
6 ZHANG Wen, SUN Yu'e, LU Guangming. Department of Thoracic Surgery, PLA General Hospital, Beijing 100853, P.R.China;The diagnostic value of determination of p16 methylation of sputum exfoliated cells for peripheral lung cancer[J];Chinese Journal of Lung Cancer;2004-01
7 Wu Xiao jun,Ding Xu hong,Hu Ke,et al Department of Respiratory Medicine,Renmin Hospital,Wuhan University,Wuhan 430060,China;The Value of Detecting Alteration Genes Alteration in Sputum Specimens for the Early Diagnosis of Lung Cancer[J];Journal of Hubei Medical University;2001-01
8 WT4”BX]GAO Zhong du,YUAN Wei xiang,MAO Ai wu,WANG Min,JIANG Ting hui [WT4”HX](Shanghai Tongren Hospital ,Shanghai 200050, China) [WT5HZ];Diagnostic value of serum CA-50 measurement in patients with carcinoma of digestive system[J];HEBEI MEDICINE;2000-04
9 YANG Liping 1) , ZHANG Shulin 2) , LIU Wanggen 3) , HE Yi 4) 1)Subject of Biochemistry and Molecular Biology, Henan Chinese Traditional Medical College,Zhengzhou 450003 2)Department of Molecular Biology,Chest Hospital of Henan Province,Zhengzhou 450003 3)Subject of Histology and Embryology, Henan Chinese Traditional Medical College,Zhengzhou 450003 4)Department of Thoracic Surgery, Henan Provincial People's Hospital, Zhengzhou 450003;Detection of aberrant methylation of p16 gene in lung tumor tissues,corresponding bronchoalveolar lavage fluid, and sputum by methylation-specific PCR[J];Journal of Zhengzhou University(Science Medical);2003-05
10 Huang Yanping, Jin Kewei, Gao Qian, Li Baogang. Department of Pathology, Kunming Medical College, Kunming 650031, China;p53 Mutation in Lung Cancer in Gejiu and Xuanwei Regions of Yunnan Province[J];Journal of West China University of Medical Sciences;2001-03
【Secondary References】
Chinese Journal Full-text Database 10 Hits
1 LV Lili,ZHU Shuyang,YANG Rongli,ZHOU Xiaobing(The Affiliated Hospital of Xuzhou Medical College,Xuzhou 221002,China);Clinical Observation on Treatment Efficacy of Malignant Pleural Effusion by the use of Integrative OK-432,Cisplatin and Mitomycin[J];Harbin Medical Journal;2008-01
2 YUAN Song-ling1,TANG Hui-huan2(1.Department of Surgery,the First Hospital of Changde,Hunan 415000;2.Department of Surgery,Xiangya Hospital,Central South University,Changsha,410008,China);The Expressions and Significance of P16. Cyclin D_1 Proteins in Cholangiocarcinoma[J];Journal of Clinical Research;2007-07
3 XU Shiyang HAN Qiang BAI Yuming,et alThe Tuberculosis Institute of Dezhou,Shandong province, Dezhou 253004, China;Clinical investigation of compound Salvia Miltiorrhiza Bunge in treating tuberculosis pleurisy[J];Journal of Clinical Pulmonary Medicine;2005-04
4 YANG Song ZhANG Yaoting YIN Jiantuan Respiratory Medicine of 175th Hospital, PLA Zhangzhou Fujian, 363000, China;A clinical analysis of 40 cases of extravasated pleural effusion[J];Journal of Clinical Pulmonary Medicine;2005-05
5 ZHANG Yihui, WANG Zeqiao, YANG Ke, et al The Department of Respiratory Diseases, The 1st People’s Hospital of Shanghai, Shanghai 200081,China;Vale of ADA in differential diagnosis of hydrothorax[J];Journal of Clinical Pulmonary Medicine;2006-04
6 LI Tiecheng;Diagnostic Significance of Interferon Gamma Concentration,Adenosinase Deaminase Activity in Tuberculous and Nontuberculous Pleural Effusions[J];Journal of Clinical Pulmonary Medicine;2007-11
7 WANG Hong-xiu,YANG Jing-hui,WANG Ying Department of Clinical Lab,Shanghai Pulmonary Hospital,Shanghai 200433;Diagnostic value of adenosine deaminase,interferon-γ and sialoid acid in pleural effusion of tuberculous and malignant tumor[J];Journal of Clinical Pulmonary Medicine;2008-06
8 LI Lue,DONG Yang-qin,Mao Yu-ping,Cheng Zhi-ping,Zhang Qi-he,Shao Yan-er. Zhoushan People′s Hospital,Zhoushan 316000,China;Differential diagnosis value of multiple indexes in pleural effusions[J];Journal of Clinical Pulmonary Medicine;2008-11
9 LUO Hong. Department of Health Care,The People's Hospital of Chengdu,Sichuan Province,Chengdu 610072,China;Test for adenosine deaminase for diagnosing tubercular pleural effusions[J];Journal of Clinical Pulmonary Medicine;2008-12
10 CHEN Quan-de The Second People's Hospital of Tongling 244000,China;Value of differential diagnosis value of multiple indexes in pleural effusions[J];Journal of Clinical Pulmonary Medicine;2009-08
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