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《CHINESE JOURNAL OF CANCER》 2000-03
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The clinical significance of deletion of p16 gene homozygous deletion in malignant pleural effusion

GUI Shuyu 1、2 , WANG Yuan1, LIU Hu1, et al 1 Laboratory of Molecular Biology and Department of Biochemistry, Anhui Medical University, Hefei 230032, PRChina 2 Department of Respiratory Medicine, the First Affiliated Hospital of Anhui Medical Uni  
Objective:To investigate the clinical significance ofhomozygous deletion of p16 gene in malignant pleural effusionMethods:The homozygous deletion of p16 gene exon1 and exon2 was examined by polymerase chain reaction (PCR) in 31 specimens of pleural effusions from lung cancer patients and compared with the determination of exfoliated cell in same specimens fromwhich the clinical value was analysed in clinical diagnosisResults:No homozygous deletion of p16 gene exon1 was found in 31 malignant pleural effusion samplesThe homozygous deletion of p16 gene exon2 was detected in 12 sampls from31 pleural effusions, while cytology positivity was found in 16 of all examined pleural effusionsThe positive rate in 31 malignant pleural effusion samplesby the determination of homozygous deletion of p16 gene and the examination of exfoliated cell was 3871%(12/31) and 5162%(16/31), respectivelyThe positive rate could be enhanced from 5162(16/31) to 7097%(22/31) by using both ofabove methods, There was no significnt difference of positive rate between homozygous deletion of p16 gene exon2 and exfoliated cytology The overall positive rate of both examination was higher than that of only the determination of homozygous deletion of p16 gene exon2 or exfoliated cytology in malignant pleural effusionNo homozygous deletion of p16 gene was found in 21 tuberculosis pleural effusion samplesConclusions:Our present data suggest that combining examinations of exfoliated cytology and homozygous deletion of p16 gene exon2 in pleural effusion can not only increase the positive rate of diagnosis, but also recruit and enhance the diagnostic value of conventional pleural cytology
【Fund】: 安徽省教委自然科学研究基金!(96-JL077);; 安徽医科大学附属医院科研基金资助
【CateGory Index】: R730.4
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