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《CHINESE JOURNAL OF CANCER》 2000-04
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Effects of etoposide on growth proliferation and apoptosis in human HIC/S cells

ZHOU Jun min, CAO Jian guo, LIAO Duan fang Department of Pharmacology, Hengyang Medical College, Hengyang 421001, P R China  
Objective:To detect the effects of etoposide on the growth,proliferation and apoptosis of HIC/S cells and its preliminary mechanism Methods:The MTT colorimetric assay was applied to examine the growth inhibition,and DNA fragmentation assay was used to observe the apoptosis induced by etoposide in HIC/S cells Expression of pRb in HIC/S cell line was proved again by immunohistochemistry and semi quantitated with Western blot analysis Results: Incubation with etoposide (0 045~28 125 μmol/L)for 48 hours significantly inhibited growth proliferation of HIC/S cells in a dose dependent manner(P 0 05) The IC 50 was 0 042±0 007 μmol/L Etoposide induced apoptosis in HIC/S cells Rb protein was expressed in HIC/S cell line and its expression was enhanced by etoposide Conclusions: Etoposide inhibites the growth proliferation and can induce apoptosis in HIC/S cell line,which is possibly related to enhanced Rb protein expression
【CateGory Index】: R735.35
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