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An in vitro study of effect of Kushen compound preparation on human colon cancer cell line SW480

ZHOU Lin, AI Zhong li, LIU Zhi su,et al Department of General Surgery of the Second Affiliated Hospital of Hubei Medical University, Wuhan 430071, P R China  
Objective: To explore the effect of Kushen compound preparation on human cancer cell line SW480 in vitro Methods: Inhibition of SW480 cell growth by Kushen compound preparation was determined by MTT assay and growth curve, and apoptosis was detected by morphological assay and flow cytometry (FCM) Results: The solution of Kushen compound preparation showed dose dependent and time dependent inhibitory effects on the proliferation in SW480 cell line The cells treated with 30 mg/ml compoumd for 48 hours showed characters of apoptosis under light and electronic microscopy Sub G 1 peak was detected by FCM, the ratio of apoptosis was 11 13% The cell percent of S phrase increased from 25 41%to 41 27%(P 0 01), and that of G 2/M phrase decreased from 6 74%to 1 35%(P 0 01) after treatment Conclusion: In vitro, Kushen compound preparation has growth inhibiting effect and may induce apoptosis in SW480 in a certain dose range, which may be one of the antineoplastic effects of Kushen compound preparation
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