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Influence of antisense bcl-2 gene on survival of mononuclear leukemia cell U937 and the sensitivity of the cell to chemotherapeutic drugs

HE Feng tian, ZHU Xi hua, HUANG Yun hui Department of Immunology, The Third Military Medical University, Chongqing 400038, P R China  
Objective:To enrich the data of tumor cell apoptosis regulated by bcl 2 and antisense bcl 2 through studying the role of antisense bcl 2 in mononuclear leukemia cell line U937 Methods:A U937 cell model (U937/as bcl 2) was established by transferring the antisense bcl 2 expression vector pLXSN/as bc1 2 into U937 cell bcl 2 expression in the model cell was detected by fluorescence activated cell sorter The model cell growth and survival were analyzed by counting the numbers of total and survival cells in normal culture condition or in the presence of Ara C or harringtonine Results:①bcl 2 expression in the model cell significantly decreased ②The model cell had a normal growth and survival in normal culture condition ③The percentage of the survival model cells significantly decreased in the presence of Ara C or harringtonine Conclusions:①Antisense bcl 2 gene transfer alone can significantly suppress bcl expression in U937 cells, but it had no obvious effect on the cell growth and survival in normal culture condition ②Antisense bcl 2 gene transfer can increase the sensitivity of U937 cell to chemotherapeutic drugs
【CateGory Index】: R733.7
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