Norcantharidin induces apoptosis in human breast cancer cell line
RONG Yu＊ , LIANG Fu you, CHEN Li, DU Hai jun, LIU Le yao, SUN Hong liu, AN Wei Department of Cell Biology, Capital University of Medical Sciences, Beijing 100054, P.R.China
Objective: The current study was designed to identify molecular mechanism of antitumor effect produced by NCTD(norcantharidin) in human breast cancer cell line. Methods: MCF-7 cells were grown in standard conditions. At the indicated time points after NCTD treatment, evaluation of apoptotic morphology by light microscopy, analysis of chromatin condensation by electron microscopy, determination of the percentage of apoptotic cells by flow cytometry and Western blot analysis for detecting of bcl-2 gene expression were conducted. Results: Differences in cellular morphology were observed between NCTD-treated and control MCF-7 cells. Cell shrinkage, cell budding and chromatin condensation were observed in the cells treated with 10μ g/ml NCTD for 12 hours. Percentage of apoptotical cells was increased while the expression of bcl-2 products was decreased with the increasing of incubation time. Conclusion: The results indicate that one of the major antitumor mechanisms of NCTD may be through inducing cell apoptosis which related to the regulation of bcl-2 gene expression.
【Fund】： 北京市自然科学基金!（ No.7973021）