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《Chinese Journal of Cancer》 2000-12
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Induction of apoptosis by trichosanthin in gastric cancer cell MKN-45

TU Shui ping* , JIANG Shi hu, QIAO Min min, WANG Li fu, ZHANG Shu, YUAN Yao zong, WU Yun li,WU Yu xin. Department of Gastroenterology, Ruijin Hospital, Shanghai Second Medical University, Shanghai 200025,P.R.China  
Objective: The current study was designed to explore the mechanism of actions of trichosanthin(TCS) on gastric cancer cell MKN 45. Methods: Cell proliferation ability, colony formating rate of gastric cancer cell MKN 45 treated by TCS were examined by using growth curve and colony formating assay. The cytotoxicity of TCS on MKN 45 cells was determined by using MTT assay. Apoptosis and cell cycle changes of MKN 45 induced by TCS were investigated by using TUNEL method and flow cytometry. Results: TCS signifcantly inhibited the growth of MKN 45. The rates of inhitition were correlated with the concentration of TCS. Colony formating rates of MKN 45 treated with 1μ g/ml TCS and 0.1μ g/ml TCS were (6.63± 1.31)% and (14.68± 1.27)% respectively, which were much lower than that of control group (23.67± 2.76)% (P
【Fund】: 卫生部消化疾病重点实验室青年基金!(No: 9806010)资助
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