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《Chinese Journal of Cancer》 2001-08
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Inhibitory Effect of Emodin on Metastasis Associated Properties of PG Cells from a Human highly Metastatic Giant Lung Carcinoma Cell Line

WANG Xin hua, ZHEN Yong su*Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, P.R.China  
Objective: To investigate the inhibitory effect of emodin, a herb derived tyrosine kinase inhibitor on metastasis associated properties of PG cells from a human highly metastatic giant lung carcinoma cell line. Methods: MTT assay was used to determine the growth inhibition by emodin in several cultured tumor cells. Invasive capacity was determined by Boyden Chamber assay. Gelatin zymography was performed to detect the secretion of MMP 2 and MMP 9 of PG cells. Flow cytometry and Western blot were applied to detect the change of cell cycle and expression level of cyclinB1 and P34cdc2 proteins. Results: Emodin inhibited the proliferation of several cultured cancer cell lines with IC50 value in range of 15 μmol/L to 70 μmol/L. After 24 h treatment, emodin reduced the invasive capacity and the secretion of MMP 2 and MMP 9 of PG cells, wherease adhesive ability of PG cells to ECM was unaffected. Emodin treatment blocked cell cycle in G2/M phase. Western blot showed down regulation of the expression level of cyclinB1 protein. Conclusion: These results suggest that emodin, which reduces the metastasis associated properties of PG cells, might be potentially useful in cancer therapy.
【Key Words】： Emodin Invasion Metastasis Type Ⅳ collagenase Cell cycle
【Fund】： 国家自然科学基金项目No:39870941
【CateGory Index】： R734.2
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