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《Chinese Journal of Cancer》 2001-08
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Glycyrrhiza uralensis Fisch Extract Selectively Induces Apoptosis in Several Human Cancer Cell Lines in Vitro

MA Jing1, FU Nai yang2, LI Yu mei3, PENG Wen lie1, XU An long1*1.Department of Biochemistry and Center for Biopharmaceutical Research, College of Life Science, Zhongshan University, Guangzhou 510275, P.R.China2.Institute for Drug Control of Guangdong  
Objective: To determine the anti tumor activity of glycyrrhiza uralensis Fisch in terms of apoptosis induction. Methods: Apoptosis was detected by fluorescence microscope and flow cytometry. Intracellular free Ca2+ and mitochondrial transmembrane potential (△Ψm) were measured by fluorescent probes Fluo 3 and Rh123, respectively. The expression of three proto oncogenes, c myc, c jun, and c fos,was examined by immunohistochemistry. Results: Glycyrrhiza uralensis Fisch extract selectively induced apoptosis in several human cancer cell lines, including MGC 803, HepG2, NSCLC, and Hela. Increase of intracellular free Ca2+ and decrease of △Ψm were observed in the apoptosis of MGC 803, HepG2, and NSCLC. Interestingly, glycyrrhiza uralensis Fisch extract couldn′t induce apoptosis in BGC-823, however, the changes of intracellular free Ca2+ and △Ψm were similar to that of apoptotic cell lines. The expression of c myc, c jun, and c fos in MGC 803 was elevated after the treatment. Conclusions: The anti tumor acitivity of glycyrrhiza uralensis Fisch is related to its ability of apoptosis induction in tumor cells. Increase of intracellular free Ca2+, decrease of △Ψm and the up regulation of c fos, c jun, and c myc protein expression take part in the regulation of apoptosis induced by the extract of glycyrrhiza uralensis Fisch.
【Fund】: 国家新药博士基金项目(No.96-901-06-36);; 广州市科委重点项目(No.97-Z-12-01)
【CateGory Index】: R73-36
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【References】
Chinese Journal Full-text Database 1 Hits
1 GAO Hong xia,SHAO Shi he,WANG Guo qing (Medical College of Beihua University,Jilin 132001,China);Research progress of glycyrrhiza uralensis fisch[J];Journal of Jinggangshan Medical College;2004-05
【Citations】
Chinese Journal Full-text Database 1 Hits
1 MA Jing1, PANG Da ben2, XU An long1*, et al 1 Department of Biochemistry and Biopharmaceutical Centre, College of Life Science, Zhongshan University, Guangzhou 510275, P R China2 Institute of Fundamental Theory Research, China Academy of Chinese Tr;Meditation of methodology for improvement on detecting DNAchange during apoptosis of MGC 803 cell line[J];CHINESE JOURNAL OF CANCER;2000-08
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 WANG yong, LIU Xingyan, GE Baofeng, et al The Institute of Traumatology and Orthopaedics,Lanzhou General Hospital, Lanzhou 730050,PRChina;Expression of c-myc, PCNA and Fas protein in osteogenic tumour[J];CHINESE JOURNAL OF CANCER;2000-03
2 Wang Yuling Li Daoming Xu Hong Tao Min Wang QingcaiFirst Affiliated Hospital of Soochow University,Suzhou 215006,China;Combination of Kanglaite injection and chemotherapy for treatment of advanced non-small cell lung cancer[J];Oncology Progress;2005-03
3 Wang Huilian,Zhou Xueping Nanjing University of TCM(210029);Review on Systemic Erythemia Lupus(SEL)Treated with TCM[J];Journal of Zhejiang University of Traditional Chinese Medicine;2007-06
4 SUN Hong dan,JIA Li,MENG Xiu xiang, et al.Dalian Medical University (Dalian,116027);STUDY ON TRADITIONAL CHINESE MEDICINE RECIPE IN INDUCING BURKITT′S LYMPHOMA CELLS-RAJI CELLS APOPTOSIS[J];Journal of Leukemia;2002-02
5 JIA Li,KONG Li,MIAO Xiao yan, et al.Dalian Medical University (Dalian,116027);SIGNIFICANCE OF P-gp bcl-2 PROTEINS EXPRESSION IN MULTIDRUG RESISTANCE OF HUMAN ERYTHOLEUKEMIA[J];Journal of Leukemia;2002-06
6 YANG Xiu Wei, ZHANG Li He (The State Key Laboratory of Natural and Biomimetic Drugs of Peking University,Beijing 100083, China);The basic and applied basic researches are the foundation of research and development of new drugs[J];Journal of Beijing Medical University;2001-03
7 WANG?Dajie(Department of Biological Engineering,Chengdu University,Chengdu 610106,China);Preliminary Analysis of Modernization of Traditional Chinese Medicine[J];Journal of Chengdu University (Natural Science);2003-01
8 JIA Li,MENG Xiu-xiang,SUN Hong-dan, SONG Zhen-lan,et al (Dalian Medical University,Dalian116027,China);Studies of BMK on inhibition of cell growth and induction of apoptosis in Yac-1 cell line[J];Journal of Dalian Medical University;2001-03
9 SUN Hua ming, CAO Jia, Michael Nusse (Molecular Toxicology Laboratory, Third Military Medical University, Chongqing 400038, China);Studies on micronucleus formations in 3 cell lines induced by Tripterygium hypoglaucun Hutch[J];Acta Academiae Medicine Militaris Tertiae;2001-03
10 AO Lin, CAO Jia, XU Ying, YANG Lu jun, ZHOU Zi yuan, LIU Sheng xue (Laboratory of Molecular Toxicology, Third Military Medical University, Chongqing 400038, China);Apoptosis of human promyelocytic leukemia HL-60 cells induced by total alkaloids of Tripterygium hypoglaucum (Level.) Hutch[J];Acta Academiae Medicine Militaris Tertiae;2001-11
【Secondary References】
Chinese Journal Full-text Database 9 Hits
1 DONG Hong~(1),ZHANG Yong-dong~(1),LIU Feng-hua~(2),X Jian-qin~(1)(1.College of Veterinary Medicine,China Agricultural University,Beijing,100094,China;2.Beijing Agricultural College,Beijing,102206,China);Advance in the Effects of Functional Regulation of Exocrine Pancreatic Secretion[J];Progress In Veterinary Medicine;2005-10
2 HU Ren-huo et al (Department of Chemistry and Life Sciences,Xianning University,Xianning,Hubei 437100);Study on Action of Bacteriostasis and Disinfection on Ten Kinds of Chinese Herbal Medicine[J];Journal of Anhui Agricultural Sciences;2009-25
3 MA Wuan-xia,HU Ren-huo,Wang Juan,FU Ying-ying,HU Jing(Xianning University and the department of Chemistry and life sciences,xianning,Xianning 437100,China);Study on Antifungal Action and Disinfection of Five Kinds of Extracting Solutions of Chinese Herb Medicine[J];Journal of Changjiang Engineering Vocational College;2009-03
4 Xingqin ZHOU,Bin JI Department of Radiotherapy,Affiliated Hospital of Nantong University,Nantong 226001,China;Current Treatment of Brain Metastases from Lung Cancer[J];Chinese Journal of Lung Cancer;2009-10
5 GONG Xue-long,LUO Ye,TANG Da-chuan,SUN Xiao-fei(1.College of Pharmacy,School of Radioactive Medicine and Public Hygiene,Department of medicine,Soochow University,Suzhou 215123,China;2.Changshu Institute of Technology,Changshu 215500,China);Research progress in glycyrrhetic acid and its derivatives[J];Strait Pharmaceutical Journal;2008-09
6 GAO Yan-li,GAO Shan-lin,JIAO Xiao-ke,ZHANG Xiao-xia,WU Jiong(China Pharmaceutical University,Nanjing 210038,China);Effect of nitrogen source on the production of flavonoids by suspension cell culture of Glycyrrhiza inflata Batal[J];Strait Pharmaceutical Journal;2008-10
7 CAO Yang,CHEN Wen-na,ZHAO Dan-yu,MA Xian-de(Liaoning Univeristy of TCM,Shenyang 110032,Liaoning,China);An Experimental Study on the Motirational Effect of Elemene on Apoptosis of the Cell A549 of Pulmonary Cancer[J];Journal of Liaoning University of Traditional Chinese Medicine;2008-04
8 XU Tao,XU Rui-an(Institute of Molecular Medicine,Huaqiao University,Quanzhou 362021,China);Progress on Ati-Tumor Mechanism of Chinese Medicine and Its Active Ingredients[J];Journal of Huaqiao University(Natural Science);2009-04
9 CHEN Yi1,HUANG Yong-huo1,KANG Xiao-bing2(1.Department of Radiology,the First Affiliated Hospital,Chongqing Medical University,Chongqing 400016,China;2.Department of Radiology,the First People's Hospital of Jiangbei,Chongqing 400020,China);Treatment of the implanted VX2 liver tumor in rabbits with percutaneous intratumor AiDi injection[J];Chinese Journal of Interventional Imaging and Therapy;2010-02
【Secondary Citations】
Chinese Journal Full-text Database 3 Hits
1 YAN Rui-qi LI Jun-li HUANG Mei-ling HUANG Xiao-yan WANG Hui-yun FENG Qi-sheng (Cancer Institute, Sun Yat-sen University of Medical Sciences. Guangzhou, 510060);PROTECTIVE EFFECT OF GLYCYRRHIZIN ON DNA REPALR DURING ITS INHIBITION ON DEN-INDUCED HEPATOCARCINOGENESIS[J];Chinese Journal of Cancer;1995-04
2 Chen Zhengming Wu Qiao Chen Yuqiang, et alThe State Lab. for Tumor Cell Engineering, Xiamen Univ, Xiamen, Fujian, 361005;The effect of retinoic acid on growth inhibition and p53、c mycexpression in gastric carcinoma cells[J];CHINESE JOURNAL OF CANCER;1998-06
3 Wang Chenglong; Wang Qiru(Experimental Hematology Laboratory, Hunan Medical Universiy;EFFECT OF HUMAN FLBROBLAST CONDITIONED MEDIUM ON THE GROWTH OF CFU-GM[J];BULLETIN OF HUNAN MEDICAL UNIVERSITY;1995-05
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