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《Chinese Journal of Cancer》 2001-08
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Regulation of G_1 Phase Cell Cycle Regulation Factors in Differentiation and Apoptosis of Matrine Induced Hepatoma Cells

SI Wei ke*, GAO Li hong, LIU Bing, CHENG An, LI Peng, YAO JieDepartment of Clinical Biochemistry, the Third Military Medical University, Chongqing 400038, P.R.China  
Objective: To observe changes of cell cycle G1 phase regulation factors during matrine induced differentiation and apoptosis in hepatoma cells, and to investigate the function of matrine in regulating proliferation of tumor cell. Method: HepG2 cells was treated for 3 days with 0, 0.3, 0.8, 1.5 g/L matrine. The protein expression of p53,p21,p27,p16,Rb,cyclin D1 was detected by immunohistochemistry and mRNA expression of p53,cyclin D1 was determined with in situ hybridization stain method. All results were analyzed quantitatively with high fidelity colored picture and statistically with Microsoft Excel Soffware. Result: After matrine treatment, the expression of cell cycle negative regulation factors p53, p21, p27, Rb, p16 were increased; while the expression of positive regulation factor cyclin D1 was decreased. Conclusion: The mechanism of matrine induced differentiation and apoptosis in HepG2 may relate to that matrine upregulate the expression of G1 cell cycle negative regulation factors and downregulate the expression of G1 cell cycle positive regulation factor cyclin D1.
【CateGory Index】: R735.7
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【References】
Chinese Journal Full-text Database 10 Hits
1 YANG Zhi-Yun1, YAO Shu-Kun1, YIN Fei1, XIU He-Ming2 1. Department of Gastroenterology, The Fourth Affiliated Hospital, Hebei Medical University, Shijiazhuang, Hebei, 050011, P. R. China 2. Central Laboratory, Bethune International Peace Hospital, Shijiazhuang, Hebei, 050082, P. R. China;Inducement Effect of Matrine on Phenotype Changes of Rat Hepatic Oval Cells[J];Chinese Journal of Cancer;2006-12
2 XU Yue1,QIN Jian2,ZHOU Yuan-Da3,HEI Hai-Xia31Department of Enterochirurgia,2Laboratory of Chemistry,Academy of Chinese Materia Medica,3Basic Medicine Project Research Lab,First Affiliated Hospital,Chongqing Medical University,Chongqing 400016,China;Study of matrine injections on colorectal carcinoma cell line LoVo transplanted subcutaneously in nude mice[J];Journal of the Fourth Military Medical University;2009-04
3 XU Yue1,QIN Jian2,ZHOU Yuan-Da3,HE Hai-Xia31Department of Enterochirurgia,Institute of Traditional Chinese Medicine,Chongqing 400013,China,2Laboratory of Chemistry,Academy of Chinese Materia Medica,Chongqing 400065,China,3Laboratory of Basic Medicine Project Research,First Affiliated Hospital,Chongqing Medical University,Laboratory of Biological Engineering,College of Preclinical Medicine,Chongqing 400016,China;Effect of proliferation and apoptosis on LoVo cells by matrine injections in vitro[J];Journal of the Fourth Military Medical University;2009-15
4 Zhi Wang,Xiang-Zhen He,Hui-Can Peng,Qi-Guo Xiao,Jia-Yi Liu Department of Ophthalmology,the Second Affiliated Hospital of Nanhua University,Hengyang 421001,Hunan Province,China;Effect of matrine on cell cycle of rabbit lens epithelial cells in vitro[J];International Journal of Ophthalmology;2010-02
5 Zhu Shi-yan1,Gan Ping2,Cao Yuan-guo1,Cheng Zhuo1(1.Department of Nephrology,Longjiang Hospital,Foshan Guangdong 528318,China;2.Department of Nephrology,Affiliated Hospital of Guiyang Medical College,Guiyang Guizhou 550001,China);Effect of matrine on high glucose-induced NF-κB p65 overexpression in rat peritoneal mesothelial cells[J];Clinical Journal of Medical Officers;2010-05
6 FU Wen-yan,LI Xia,WANG Xue-fei,YANG Ying (College of Animal Science and Veterinary Medicine,Inner Mongolia Agricultural University,Hohhot 010018,China);Progress of Alkaloid of Sophora alopecuroides L.Act in Anti-tumor[J];Animal Husbandry and Feed Science;2009-10
7 ZHAO Si-wei1,2,YU Miao1,2,3,BAI Yun-jiao1,2,CHANG Kai-lin1,2,CHEN Ying-xiang1,2,JI Yu-bin1,2,3(1.Research Center on Life Sciences and Environmental Sciences,Harbin University of Commerce,Harbin 150076,China; 2.Engineering Research Center of Natural Anticancer Drugs,Ministry of Education,Harbin 150076,China; 3.Engineering Laboratory on Natural Durgs and Biomimetic Drugs,Heilongjiang Development and Reform Committee,Harbin 150076,China);Study on anti-tumour activity of natural alkaloids[J];Journal of Harbin University of Commerce(Natural Sciences Edition);2012-05
8 ZHU Dan-dan,YAO Shu-kun,YAN Jian-guo,PAN Lin,LI Hong Department of Medical Gastroenterology,Peking University,China-Japan Friendship Hospital,Beijing 100028,P.R.China;Prevention and treatment of matrine on hepatic carcinoma of rat and its mechanism[J];Chinese Journal of Cancer Prevention and Treatment;2010-04
9 HU Mei-jie, ZENG Hui,ZHANG Shu, ZHANG Xue-jun, ZHANG Yong-ping, QIAO Min-min, FU Hua, WU Yun-lin (Department of Gastroenterology, Ruijin Hospital, Shanghai Second Medical University, Shanghai 200025, China);Mechanisms Underlying Apoptosis of Gastric Cancer Cell Line Induced by Combination of 5-Fluorouracil and Matrine[J];Acta Universitatis Medicinalis Secondae Shanghai;2005-04
10 CHEN Jian Shanghai General Armed Force Hospital;Progress of Experimental Research of Radix Sophorae Flavescentis in Treating Liver Cancer[J];Shanghai Journal of Traditional Chinese Medicine;2006-01
【Citations】
Chinese Journal Full-text Database 2 Hits
1 SI Wei ke, XIAO Tao yuan, KANG Ge fei (Department of Clinical Laboratory Medicine, Third Military Medical University, Chongqing 400038, China);Effects of matrine on cell morphology and related factors of hepatocellular carcinoma cell HepG_2 in vitro[J];ACTA ACADEMIAE MEDICINE MILITARIS TERTIAE;2000-06
2 Wei Ke Si~1, Jing Pan~2, Hua Lu~2 and Zhao Quan Li~2 1 Department of Clinical Biochemistry, Third Military Medical University, Chongqing 400038 China 2 Department of Laboratory Medical Science, Third Military Medical University, Chongqing 400038 China;Study on matrine inhibiting proliferation of HepG2 cell and the relation between its dosage and inhibiting style[J];World Chinese Journal of Digestology;2001-02
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 WU Liang fa (General Workers' Hospital of Anhui Tongling Nonferrous Metal Company,Tongling 244000,China);Comparision of therapeutic effects and cost-effectiveness analysis of matrine and α-interferon for chronic hepatitis B[J];Anhui Medical and Pharmaceutical Journal;2004-05
2 WANG Yong,WU Yu-lian,LIU Ying-bin,TANG Zhe(Department of General Surgery,Second Affiliated Hospital of Zhejiang University,Hangzhou 310009,China);Experimental study on differentiation of human hepatocellular carcinoma cell line Bel-7404 induced by matrine in vitro and its mechanism[J];China Journal of Traditional Chinese Medicine and Pharmacy;2009-07
3 CUI Ying,ZHANG Yi,HU Chun-mei(College of Bio-engineering,Chongqing Institute of Technology,Chongqing 400050,China);Extraction and Separation of Sophora Flavescens Alkaloid[J];Journal of Chongqing Institute of Technology(Natural Science Edition);2007-03
4 Cai Xiong; Wang Guojun; Qu Yao; Fan Chenghui; Zhang Ruiqi; Xu Wensheng (Department of Infectious Diseases, Changzheng Hospital, Second Military Medical University,Shanghai, 200003);Clinical efficacy of kurorinone in the treatment of chronic hepatitis B[J];Academic Journal of Second Military Medical University;1997-01
5 SI Wei ke, CHENG An, LI Peng, LIU Bing, GAO Li hong, YAO Jie (Department of Clinical Biochemistry, Third Military Medical University, Chongqing 400038,China);Study on apoptosis of human hepatoma cell line HepG_2 induced by matrine[J];Acta Academiae Medicine Militaris Tertiae;2001-07
6 SI Wei ke, LI Peng, YAO Jie (Department of Clinical Biochemistry, Third Military Medical University, Chongqing 400038, China);Effects of matrine on HepG_2 cells at metabolism level and gene level[J];Acta Academiae Medicine Militaris Tertiae;2002-11
7 TANG Zhong zhi, FU Li bo, TANG Ying (Wuhan General Hospital, Guangzhou Command of PLA, Wuhan 430070, China);Inhibition of growth and induction of apoptosis in human hepatoma cell line by tanshinone ⅡA[J];Acta Academiae Medicinae Militaris Tertiae;2003-09
8 ZHANG Yong qing, HUANG Gao shen, WANG Zhe, FENG Ji liang, GUO Ying (Department of Hematology, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China);Study of the mechanism of anti-leukemia proliferation of matrine with cDNA array[J];Acta Academiae Medicinae Militaris Tertiae;2003-14
9 WANG Yuan, SI Wei ke, LI Peng, YAO Jie (Department of Clinical Biochemistry, College of Medical Laboratory, Third Military Medical University, Chongqing 400038, China);Effects of matrine and oxymatrine on the proliferation and the apoptosis of A549 cells[J];Acta Academiae Medicinae Militaris Tertiae;2004-09
10 YANG Ze-Song1,MU Jun2,CHEN Jian-Bin1,ZHANG Hong-Bin1,HU Ni-Ni1,HUANG Zong-Gan11Department of Haematology,2Department of Neurology,First Affiliated Hospital,Chongqing Medical University,Chongqing 400016,China;U937 cell apoptosis-inducing effects of matrine and its effect on Bcl-2 expression[J];Journal of the Fourth Military Medical University;2008-24
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 XIANG Hua\+1,ZHAO Jian-zhong\+2, BO Lin\+2 (1.Hygiene and Anti-epidemic Station of Hangzhou, Hangzhou\ 310006, China; 2.Department of Cytobiology, College of Biological Sciences, Zhejiang University, Hangzhou 310009, China);STUDY ON THE ANTIMUTAGENICITY OF SEAWEED POLYSACCHARIDES[J];Carcinogenesis,Teratogenesis and Mutagenesis;2001-01
2 ZHANG Chun_ling, HU Jun_feng, QU Jiang_bin, et al.(School of Public Health, Shandong University, Jinan 250012 , Ch ina);STUDY ON ANTIMUTAGENICITY OF CHINESE HERBAL MEDICINE AND GREEN TEA TO BENZOAPYRENE AND NNK[J];Carcinogenesis,Teratogenesis and Mutagenesis;2003-02
3 ZHANG Jing 1 ,SHAN Bao-en 1, ,LIU Gang-san 2 , ZHAO Xue-tao 1 ,CHEN Shu-hong 2 (1.Research Center,the Fourth Hospital of Hebei Medical University,Shijiazhuang050011,Hebei,China;2.New Drug Research and Development Co.,Ltd.of North China Pharmaceutical Corporation,Shijiazhuang050015,Hebei,China);Anti-tumor Activity of Extract from Periploca Sepium Bge[J];Carcinogenesis,Teratogenesis & Mutagenesis;2006-02
4 LIU Min-sheng1, REN Ze-ming2, WEI Xue-tao2,(1. Department of Otorhinolaryngology, Lanzhou Petrochemical Hospital, Lanzhou 730060, Gansu; 2. Department of Toxicology, Peking University, Beijing 100191, China);Apoptosis and Caspase-3 changes induced by matrine in CNE2 cells[J];Carcinogenesis,Teratogenesis & Mutagenesis;2010-02
5 Du Yangyang and Mo ZiyaoGuangzhou Anti-epidemic Station,Guangzhou 5l0080;STUDY ON THE MUTAGENIC AND ANTIMUTAGENIC POTEN-TIALS OF GYPENOSIDES(GPS)IN MICE[J];CARCINOGENESIS, TERATOGENESIS AND MUTAGENESIS;1995-03
6 QU Xin-hua(Experimental Middle School of Hengyang in Hunan,Hengyang,Hunan 421001);Extraction and Separation Technique of Essential Oil from Plants[J];Journal of Anhui Agricultural Sciences;2007-32
7 ZHANG Xiao-le et al(College of Veterinary Medicine,Sichuan Agricultural University,Ya'an,Sichuan 625014);Study on the Preparation of Emulsion and Spiritus of the Volatile Oil from the Leaf of Cinnamomum longepaniculatum(Gamble) N.Chao and Their Sterilizing Effect to Air[J];Journal of Anhui Agricultural Sciences;2011-21
8 YAN Rui-qi LI Jun-li HUANG Mei-ling HUANG Xiao-yan WANG Hui-yun FENG Qi-sheng (Cancer Institute, Sun Yat-sen University of Medical Sciences. Guangzhou, 510060);PROTECTIVE EFFECT OF GLYCYRRHIZIN ON DNA REPALR DURING ITS INHIBITION ON DEN-INDUCED HEPATOCARCINOGENESIS[J];Chinese Journal of Cancer;1995-04
9 ZHAO Wei hong, CHEN Jia pei, CONG Yu wen,et alInstitute of Radiation Medicine, Beijing 100850,P.R.China;Caffeine promoting tumor cells apoptosis induced by irradiation[J];CHINESE JOURNAL OF CANCER;1999-04
10 WANG Jun jie, YU Li zhang, SHEN Wen jiang, et al The First Teaching Hospital of Beijing Medical University,Beijing 100034,P R China$$$$;The study of the influence of Kang Lai Te injection on radiosensitivity of renal carcinoma cell lines and its mechanism[J];CHINESE JOURNAL OF CANCER;1999-06
China Proceedings of conference Full-text Database 1 Hits
1 Lisheng Wang1,*,Yejun You1,Songqing Wang1,Xu Liu1,Buming Liu2,Xiao Lin2,Mingsheng Chen2 1School of Chemistry and Chemical Engineering,Guangxi University,Nanning,530004 2Guangxi Institute of Traditional Medical and Pharmaceutical Sciences,Nanning 530022;Synthesis,Characterization and in vitro Anti-tumor Activities of Matrine Derivatives[A];[C];2012
【Secondary References】
Chinese Journal Full-text Database 10 Hits
1 LIU Min-sheng1, REN Ze-ming2, WEI Xue-tao2,(1. Department of Otorhinolaryngology, Lanzhou Petrochemical Hospital, Lanzhou 730060, Gansu; 2. Department of Toxicology, Peking University, Beijing 100191, China);Apoptosis and Caspase-3 changes induced by matrine in CNE2 cells[J];Carcinogenesis,Teratogenesis & Mutagenesis;2010-02
2 TANG Mei-qiong et al(Guangxi Botanical Garden of Medicinal Plants,Nanning,Guangxi 530023);Study on Genomic DNA Extraction Method for Genuine Medicinal Material in Guangxi Sophora tonkinensis Gagnep[J];Journal of Anhui Agricultural Sciences;2010-17
3 ZHANG Jia-ming et al(School of Life Science,Shanxi Normal University,Linfen,Shanxi 041004);Study on Antibacterial Activity of Alkaloids from Elaeagnus mollis′s Diels[J];Journal of Anhui Agricultural Sciences;2010-17
4 CHEN Jia-yu et al(Department of Biochemistry,Zunyi Medical College,Zunyi,Guizhou 563003);The in vitro Anti Hptocarcinoma Effects of Human Interleukin-12 from Transgenic Potato[J];Journal of Anhui Agricultural Sciences;2010-21
5 SHANG Xi-yu (Nanyang Medical School,Nanyang,Henan 473000);Research on the Distribution of the Alkaloids in the Different Parts of the Dendrobium candidum with Various Origins[J];Journal of Anhui Agricultural Sciences;2010-23
6 LIU Li-hong et al (College of Chemistry and Chemical Engineering,Ningxia Normal University,Guyuan,Ningxia 756000);Optimization of Microwave Extraction Process of Total Alkaloid from Aconitum flavum[J];Journal of Anhui Agricultural Sciences;2010-36
7 LIU Hai-feng et al (Agricultural College of Yanbian University,Yanji,Jilin 133000);Insecticidal Activity of Alkaloid Extracts from Poisonous Plants[J];Journal of Anhui Agricultural Sciences;2010-36
8 YANG Yu-qin et al(Guiyang College of Traditional Chinese Medicine,Guiyang,Guizhou 550002);Contents of Total Alkaloid in Three Formulations of Sini Decoction[J];Journal of Anhui Agricultural Sciences;2011-30
9 NIU Wei et al(Research Institute of Horticultural Products Transport and Preservation,College of Horticulture,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002);Study on the Extraction Method of Alkaloids from Narcissus tazetta var.chinensis Roem[J];Journal of Anhui Agricultural Sciences;2012-19
10 QIN Kun-ming~1,LI Xiao~1,XU Zhao~2,CAI Bao-chang~(1)(1.Nanjing University of Chinese Medicine,Nanjing 210046,China;2.China Pharmaceutical University,Nanjing 210009,China);A Survey of the Studies on Pharmacological Effects of Lycorine and Its Derivatives[J];Journal of Beijing Union University(Natural Sciences);2009-01
China Proceedings of conference Full-text Database 4 Hits
1 Fang Yuan1a,Jie Chen 2a,Wen-jin Wu 3,Su-zhen Chen 1,Xue-ding Wang 1 and Min Huang 1 1 Institute of Clinical Pharmacology,School of Pharmaceutical Sciences,Sun Yat-sen University,74 Zhongshan Road II,Guangzhou 510080,P.R.China,and 2 Department of Pharmacy,the First Affiliated Hospital,Sun Yat-sen University,58 Zhongshan Road II,Guangzhou 510080,China,and 3 Institution of Chinese Medical Science,University of Macau,Av.Padre Tomás Pereira S.J.,Taipa,Macao,P.R.China.;Effects of matrine and oxymatrine on catalytic activity of cytochrome P450s in rats[A];[C];2011
2 WANG Yanhua, YIN Ying, CHU Jianjie, WENG Yan, ZHU Yanrong, GUO Chao, GUAN Yue, WEI Guo, DUAN Jialin, QUAN Wei, Zhou Dan, XI Miaomiao,WEN Aidong (Department of Pharmacy, the First Affiliated Hospital of Fourth Military Medical University, Xi’an,710032, China);Traditional Chinese Medicine for Prevention and Therapy of Coronary Heart Disease[A];[C];2013
3 LAI Yun;CHEN Zi-jun;Shanghai University of Traditional Chinese Medicine;;Progress on the Effect of traditional Chinese Medicine on the Tumor Microenvironment[A];[C];2014
4 LI Guang-lei;LIN Qing-hua;CHANG Guo-ping;LIU Bo-xuan;WU Jing-yi;YU Hui-pan;ZHANG Hong-gui;School of Chinese Pharmacy,Beijing University of Chinese Medicine;;Research advances on Pharmacology and Toxicology of Radix Sophorae subprostratae[A];[C];2013
【Secondary Citations】
Chinese Journal Full-text Database 2 Hits
1 Hang Zhenbiao 1 Wei Yuquan 1 Zhao Xia 2 1.Department of Pathology ( Chengdu 610041); 2.Department of Gynecology and Obstetrics;Electron Microscopic Observation of Hepato cellular Carcinoma in 15 Cases An Ultrastructural Comparison with Human Embryonic Liver[J];JOURNAL OF WEST CHINA UNIVERSITY OF MEDICAL SCIENCES;1996-03
2 SS Zhang, ZH Li and SF Liu (Department of Pharmacology, Neimenggu Medical College, Huhehaote 010059);RELATIONSHIP BETWEEN THE POSITIVE INOTROPIC ACTION OF MATRINE AND EXTRACELLULAR CALCIUM[J];Acta Pharmaceutica Sinica;1990-08
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