Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Chinese Journal of Cancer》 2001-10
Add to Favorite Get Latest Update

Initiated Cancerazation Effect of Reactive Oxygen Species and Its Produ cts of Oxidative DNA Damage in B EP2D Cells

ZHU Mao-xiang * ,YANG Zhi-hua,GONG Yi-fen,LU Ying,CAO Zhen-shan Institute of Radiatio n Medicine,Academy of Military Medical Science,B eijing 100850,P.R.Ch ina  
Objective:To investigate oxidative DNA damage through reactive o xygen species(ROS)produced by 60 Co gamma ray irradiation or 4-(methylnitrosamino)-1-(3-pyridyl) -1-butanone(NNK)treatment in HPV-16immortalized hu man bronchial epithel ial cell(BEP2D),and their effect in initiating cancerazation.Methods :Ethi dium bromide and 2',7'-dichlorofluorescein,fluorescent products of the mem brane-permeable dyes hydroethine and 2' ,7' -dichloroflurescin diacetate,w ere used to monitor the i ntracellular production of hydrogen peroxides(H 2 O 2 )and superoxide anions(O 2 )respectively,by flow cytometry.8-hydroxydeoxygunosine(OH8dG)was e xamined with HPLC-ECD from extr acted DNA.DNA from BEP2D irradiated by 60 Co gamma ray or treated with NNK were t ransferred into receptor C3H10T1 /2cells together with coli-neo plasmid thro ugh liposome,and positive clone of t ransfection was selected with Geneticin(G418).Cell transformation fre quency was a ssayed by counting clone formation i n semisolid agar,and carcinog enesis was evaluated by tumor formation in nude mice.Results:The ROS products and DNA adduct OH8dG in 60 Co gamma ray irradiated or NNK-treated BEP2D cells increased remarkably,a nd showed better dose-response correlation.After 60 Co gamma rays irradiated or NNK-treate d DNA transfection,the receptor C 3H10T1/2cells displayed the characte ristics of transformation,and tumor formatio n was observed in nude mice.Conclusion:Intracellular production of RO S and oxidative DNA damage induced by radi ation and /or chemical carcinogen ma y initiate ce ll cancerazation.
【Fund】: 国家自然科学基金资助项目(批准号:39700030)
【CateGory Index】: R730.2
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
Chinese Journal Full-text Database 9 Hits
1 FU Xiao-Yuan1, BIN Xiao-Nong2, TAN Min31. Fuyong Hospital, Shenzhen, Guangdong, 518101, P.R.China2. Institute of Chemical Carcinogenesis, Guangzhou Medical College, Guangzhou, Guangdong, 510182, P.R.China3. Department of Pathology, The Second Affiliated Hospital, Guangzhou University of Traditional Chemical & Medical, Guangzhou, Guangdong, 510120, P.R.China;Expressions of 8-OH-dG, k-ras, and p53 Genes in Pleural Effusion Cells and Their Clinical Significances[J];Chinese Journal of Cancer;2005-03
2 LU Jia-chun ,WU Zhong-liang ,CHEN Jia-kun ,JI Wei-dong ,SHI Lu-yuan (The Institute for Chemical Carcinogenesis, Guangzhou Medical College, Guangzhou 510182; Huazhong Univ. of Science & Technology, Tongji Medical College, Wuhan 430030 );A Study on the Role of Oxidative DNA Damage in Development of Human Lung Cancer[J];Academic Journal of Guangzhou Medical College;2003-02
3 LIU Hai-yun,TONG Jian,LI Jian-xiang*(Department of Health Toxicology,School of Radiology and Public Health,Medical College of Soochow University,Suzhou,Jiangsu 215123,China);Differences in Oxidative Damage and Gene Mutation between Smoking and Radon-induced Lung Cancer[J];Journal of Environmental & Occupational Medicine;2009-04
4 LIN Hong, HUANG Jinxing, LIU Jiying, et al. TianJin Medical University, Tianjin 300203, China;Study on the relationship between urine 8-hydroxy-2-deoxyguanosine and cancer[J];Shanghai Journal of Medical Laboratory Sciences;2003-01
5 Xiao Can,Zhou Jianhua,Hui Jianhua,et al.Department of Stomatology,The First Affiliated Hospital of Soochow University,Suzhou 215006,China;Study on the genetic toxic effect of smoking on human oral mucosa[J];Journal of Practical Stomatology;2007-03
6 Zhang Deli Zhu Shengji Luo Guangfu Huang Yingping Yuan Ding Lui Liming (Center of Ecology & Environmental Science, China Three Gorges Univ.,Yichang 443002,China);Development of Free Radical-Induced Oxidative Damage to DNA[J];Journal of University of Hydraulic and Electric Engineering/yichang;2004-06
7 Wang Yunnan, Lu Jiachun, Zeng Bohang, et al. Department of Tumor, Guangzhou Chest Hospital(510095 Guangzhou);The role of 8-hydroxydeoxyguanine in the development of human lung cancer and in the carcinogenesis of human bronchial epithelial cells[J];Clinical Medicine of China;2004-02
8 FU Xiao-yuan1,BIN Xiao-nong2,TAN Min3(1.Fuyong Hospital of Shenzhen,Shengzhen 518101,China;2.Institute for chemical Carcinogenesis,Guangzhou Medical College,Guangzhou 510182,China;3.The Second Affiliated Hospital of Guangzhou University of TCM,Guangzhou 510120,China);The Significance of Expression of 8-OH-dG and p53 Gene in Pleural Effusion Cells[J];Bulletin of Chinese Cancer;2004-11
9 FU Xiao-yuan1,TAN Min2,BIN Xiao-nong3(1.Fuyong Hospital,Shenzhen,Guangdong518103,P.R.China;2.Cancer Research Center of Guangzhou Medical University,Guangzhou,Guangdong512130,P.R.China;3.Traditional Medical University of Guangdong,Guangzhou,Guangdong510130,P.R.China);Significance of diagnosis on8-OH-dG and k-ras gene mutation from pleural cell of lung cancer[J];China Journal of Modern Medicine;2004-14
Chinese Journal Full-text Database 10 Hits
1 ZHANG Hai tao, ZHU Qi feng, MO Li er, ZHUANG Hai qi, Cai Chun (Institute of Medical Biochemistry, Guangdong Medical College, Zhanjiang 524023, China);STUDY OF EFFECT OF H_2O_2 ON CONTENT OF 8-HYDROXYGUANINES IN HL-60 CELLULAR DNA[J];CARCINOGENESIS, TERATOGENESIS AND MUTAGENESIS;2000-01
2 MENG Zi qiang\+1, ZHANG Lian zhen\+2 (1. Institute of Environmental Medicine and Toxicology, Shanxi University, Taiyuan 030006,China; 2. China Institute for Radiation Protection, Taiyuan 030006, China);DNA OXIDANT DAMAGE INDUCED BY TOBACCO SMOKE IN HUMAN BLOOD LYMPHOCYTES[J];Carcinogenesis,Teratogenesis and Mutagenesis;2001-03
3 JI Wei-dong, WU Zhong-liang, CHEN Jia-kun(Institute for Chemical Carcinogenesis of Guangzhou Medical College, Guangzhou 510182, China);MALIGNANT TRANSFORMATION OF HUMAN BRONCHIAL EPITHELIAL CELL LINE INDUCED BY CRYSTALLINK NICKEL SULFIDE[J];Carcinogenesis,Teratogenesis and Mutagenesis;2002-01
4 WANG Wei 1,2 ,WU Yong-jun 1 ,WU Yi-ming 1,ˇ (1.Department of Occupational Health and Health Toxicology,College of Public Health,Zhengzhou University,Henan Zhengzhou450052, China;2.Zhengzhou Institute of Occupational Disease, Zhengzhou Henan450053,China);Genetic Polymorphism in hOGG1 and Susceptibility to Lung Cancer[J];Carcinogenesis,Teratogenesis and Mutagenesis;2005-02
5 Ke Yue bin ,Zhang QiaoResearch Unit of Genotoxicology, Sun Yat -Sen Umiversity of Medical sciences,GuangZhou510089;STUDIES ON OXIDATIVE DNA DAMAGE AND PROTECTIONTHE BIOMARKER oH8dG[J];CARCINOGENESIS, TERATOGENESIS AND MUTAGENESIS;1995-01
6 Ma Aiguo 1,Andrew R.Collins 2,Susan J.Duthie 2 1Department of Human Nutrition,Qingdao Medical College,Qingdao 266021, 2The Rowett Research Institute,U.K.;THE DIFFERENCES OF DNA DAMAGE AND REPAIR BETWEEN SEVERAL TYPES OF CELLS[J];CARCINOGENESIS, TERATOGENESIS AND MUTAGENESIS;1997-03
7 ZHU Mao xiang1* , ZHANG Jin song2, YANG Zhi hua1, et al. 1.Institute of Radiation Medicine, Academy of Military Medicine Science, Beijing 100850, P.R. China 2.College of Economy& Technology, University of Chinese Science and Technology, Hefei 230052, P;Nanoselenium prevents lung tumorigenesis induced by 4- (methylnitrosamino)-1-(3- pyridyl)-1-butanone in Kunming mice[J];CHINESE JOURNAL OF CANCER;2000-10
8 CAO Su- mei1, LIU Qing2* , HUANG Qi- hong3, YANG Cheng- wu2, HUANG Teng- bo1 1.Department of Cancer Epidemiology, Cancer Center, Sun Yat- sen University of Medical Sciences, Guangzhou 510060, P.R.China 2.Department of Statistics, Sun Yat- sen U;Analysis for risk factors of nasopharyngeal carcinoma in Sihui City[J];CHINESE JOURNAL OF CANCER;2000-11
9 SONG Chun-ying, TAN Wen, LIN Dong-xin (Department of Etiology & Carcinogenesis, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, P. R. China);Polymorphisms of DNA Repair Gene XRCC1 in Chinese Population and its Relation to Esophageal Squamous Cell Carcinoma[J];Chinese Journal of Cancer;2001-01
10 GE Shi li, LOU Tie zhu, XIANG Xiao qiong, CHEN Wei, WU De chang*Department of Molecular Toxicology, Beijing Institute of Radiation Medicine,Beijing 100850, P.R.China;Malignant Transformation of BEP2D Cell Induced by α Particles[J];Chinese Journal of Cancer;2001-09
【Secondary References】
Chinese Journal Full-text Database 6 Hits
1 LI Yong,KONG Ling-qing,GAO Hong,YAN Yu-lin(College of Animal Science,Yunnan Agriculture University,Kunming,Yunnan,650201,China);Progress on Free Radical and Diseases[J];Progress in Veterinary Medicine;2008-04
2 Xiaobo CHEN, Yunchao HUANG Department of Thoracic Cardiovascular Surgery, the third Affiliated Hospital of Kunming Medical College(YUNNAN Tumor Hospital) , Kunming 650118, China;Advances of 8-hydroxy-2-deoxyguanosine Expression in Lung Cancer[J];Chinese Journal of Lung Cancer;2009-01
3 ZHAO Jinlian1,ZENG Youwei1,2,LI Kuan1,PENG Yonghong1,3(1.School of Life Science,Guangdong Key Lab of Biotechnology for Plant Development,South China Normal University,Guangzhou 510631,China;2.Department of Food and Bio-engineering,Guangdong Light Industry Technical College,Guangzhou 510300,Guangdong China;3.Department of Life Science,Huizhou University,Huizhou,Guangdong 516007,China);STUDY ON PROTECTION OF FLOWER TEA ON ·OH-INDUCED DNA DAMAGE THROUGH FLUORESCENT SPECTRUM SCANNING[J];Journal of South China Normal University(Natural Science Edition);2010-03
4 HUANG Yuan-Jiao,MA Ning,XUAN Chao,ZHANG Bei-Bei,FENG Hai-Yan,MURATA Mariko(Medical Scientific Research Center,Guangxi Medical University,Nanning 530021,China);Detection of 8-Nitroguanine and 8-Hydroxy-2'-deoxyguanosine,Biomarkers of Oxidative DNA Damage in Nasopharyngeal Carcinoma Tissue and Its Relationship with iNOS[J];Chinese Journal of Biochemistry and Molecular Biology;2009-04
5 LIU Sheng-lin.(The Department of Examination,Xiqing Hospital in Tianjin,300380,China);The Progress of 8-hydroxy-2′-deoxyguanosine Detecting Method[J];Medical Recapitulate;2006-17
6 JIA Hai-yan,LI Jin-min,Yu Qian,Wang Jun-jie,LI Shu(College of Veterinary Medicine,Northeast Agricultural University,Harbin 150030,China);THE EFFECT OF COLD STRESS ON DNA OXIDATIVE DAMAGE OF LUNG INCHICKEN[J];Chinese Journal of Applied Physiology;2009-03
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved